Application of six hepatitis C virus genotyping systems to sera from chronic hepatitis C patients in the United States

J Y Lau, M Mizokami, J A Kolberg, G L Davis, L E Prescott, T Ohno, R P Perrillo, K L Lindsay, R G Gish, K P Qian, M Kohara, Peter Simmonds, M S Urdea

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Serum samples from 139 US patients with chronic hepatitis C virus (HCV) infection were studied using six different genotyping systems, including both molecular and serologic methods, to determine the applicability of these approaches and the prevalence of various HCV subtypes. The concordance of genotyping results based on the various systems (except for core polymerase chain reaction genotyping) was good (93.5%). Subtypes 1a and 1b were prevalent (37.4%). Subtypes 2a (2.2%), 2b (8.6%), and 3a (5.8%) were less common. HCV genotypes could not be determined in 3.4%-16.5% of samples depending on the method used. HCV type 2 was associated with greater histologic activity but lower serum HCV RNA levels (P <.05), whereas type 3 was associated with lower serum alanine aminotransferase levels (P <.05). These data demonstrate a high concordance between HCV genotyping systems and provide a foundation for comparison of genotyping data between studies using different systems. HCV types 1a and 1b are both prevalent in the United States.
Original languageEnglish
Pages (from-to)281-9
Number of pages9
JournalThe Journal of Infectious Diseases
Issue number2
Publication statusPublished - Feb 1995


  • Adult
  • Aged
  • Alanine Transaminase
  • Antibodies, Viral
  • California
  • Chronic Disease
  • Female
  • Florida
  • Genotype
  • Hepacivirus
  • Hepatitis C
  • Humans
  • Immunoenzyme Techniques
  • Interferon-alpha
  • Male
  • Middle Aged
  • Missouri
  • Polymerase Chain Reaction
  • RNA, Viral
  • Reproducibility of Results
  • Viral Nonstructural Proteins
  • Virology


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