Projects per year
production of aquaculture species. The uptake of genomic technology in aquaculture breeding has traditionally lagged behind terrestrial farmed animals. However, the rapid development and application of sequencing technologies has allowed aquaculture to narrow the gap, leading to substantial genomic resources for all major aquaculture species. While high-density single-nucleotide polymorphism (SNP) arrays for some species have been developed recently, direct genotyping by sequencing (GBS) techniques have underpinned many of the advances in aquaculture genetics and breeding to date. In particular, restriction-site associated DNA sequencing (RAD-Seq) and subsequent variations have been extensively applied to generate population-level SNP genotype data. These GBS techniques are not dependent on prior genomic information such as a reference genome assembly for the species of interest. As such, they have been widely utilized by researchers and companies focussing on nonmodel aquaculture species with relatively small research communities. Applications of RAD-Seq techniques have included generation of genetic linkage maps, performing genome-wide association studies, improvements of reference genome assemblies and, more recently, genomic selection for traits of interest to aquaculture like growth, sex determination or disease resistance. In this review, we briefly discuss the history of GBS, the nuances of the various GBS techniques, bioinformatics approaches and application of these techniques to various aquaculture species.
- next-generation sequencing
- restriction-site associated DNA
- selective breeding
- single nucleotide polymorphism
FingerprintDive into the research topics of 'Applications of genotyping by sequencing in aquaculture breeding and genetics'. Together they form a unique fingerprint.
- 5 Finished
RCUK-CONICYT: Utilising functional genomic variation for improved disease resistance in Chilean salmon aquaculture
Houston, R. & Hickey, J.
1/02/16 → 30/11/18
1/05/15 → 30/04/17
RTD budget: FISHBOOST - Improving European aquaculture by advancing selective breeding to the next level for the six main finfish
1/02/14 → 31/01/19