Architecture of the Mouse Brain Synaptome

Fei Zhu; Mélissa Cizeron; Zhen Qiu; Ruth Benavides-Piccione; Maksym V. Kopanitsa; Nathan G. Skene; Babis Koniaris; Javier DeFelipe; Erik Fransén; Noboru H. Komiyama; Seth G.N. Grant

doi:10.1016/j.neuron.2018.07.007

Architecture of the Mouse Brain Synaptome

Fei Zhu, Mélissa Cizeron, Zhen Qiu, Ruth Benavides-Piccione, Maksym V. Kopanitsa, Nathan G. Skene, Babis Koniaris, Javier DeFelipe, Erik Fransén, Noboru H. Komiyama, Seth G.N. Grant

Research output: Contribution to journal › Article › peer-review

Abstract

Synapses are found in vast numbers in the brain and contain complex proteomes. We developed genetic labeling and imaging methods to examine synaptic proteins in individual excitatory synapses across all regions of the mouse brain. Synapse catalogs were generated from the molecular and morphological features of a billion synapses. Each synapse subtype showed a unique anatomical distribution, and each brain region showed a distinct signature of synapse subtypes. Whole-brain synaptome cartography revealed spatial architecture from dendritic to global systems levels and previously unknown anatomical features. Synaptome mapping of circuits showed correspondence between synapse diversity and structural and functional connectomes. Behaviorally relevant patterns of neuronal activity trigger spatiotemporal postsynaptic responses sensitive to the structure of synaptome maps. Areas controlling higher cognitive function contain the greatest synapse diversity, and mutations causing cognitive disorders reorganized synaptome maps. Synaptome technology and resources have wide-ranging application in studies of the normal and diseased brain.

*NOTE: Fei Zhu, Mélissa Cizeron and Zhen Qiu contributed equally.

Original language	English
Pages (from-to)	781-799.e10
Number of pages	29
Journal	Neuron
Volume	99
Issue number	4
Early online date	2 Aug 2018
DOIs	https://doi.org/10.1016/j.neuron.2018.07.007
Publication status	Published - 22 Aug 2018

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Molecular synapse imaging technology and resources
Grant, S.
UK-based charities
1/02/17 → 1/06/21
Project: Research
Lab and animal colony management
McLaughlin, C.
9/06/14 → 1/09/18
Project: Research

Synaptome data with individual synapse parameters, types and subtypes across the 5 coronal mouse brain section
Grant, S. (Creator), Koniaris, B. (Creator) & Qiu, R. (Creator), Edinburgh DataShare, 20 Jun 2019
DOI: 10.7488/ds/2366
Dataset

Seth Grant
- seth.granted.acuk
- Deanery of Clinical Sciences - Personal Chair of Molecular Neuroscience
- Centre for Clinical Brain Sciences
- Edinburgh Neuroscience
Person: Academic: Research Active
Noboru Komiyama
- Deanery of Clinical Sciences - Senior Lecturer
- Centre for Clinical Brain Sciences
- Edinburgh Neuroscience
Person: Academic: Research Active

Cite this

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title = "Architecture of the Mouse Brain Synaptome",

abstract = "Synapses are found in vast numbers in the brain and contain complex proteomes. We developed genetic labeling and imaging methods to examine synaptic proteins in individual excitatory synapses across all regions of the mouse brain. Synapse catalogs were generated from the molecular and morphological features of a billion synapses. Each synapse subtype showed a unique anatomical distribution, and each brain region showed a distinct signature of synapse subtypes. Whole-brain synaptome cartography revealed spatial architecture from dendritic to global systems levels and previously unknown anatomical features. Synaptome mapping of circuits showed correspondence between synapse diversity and structural and functional connectomes. Behaviorally relevant patterns of neuronal activity trigger spatiotemporal postsynaptic responses sensitive to the structure of synaptome maps. Areas controlling higher cognitive function contain the greatest synapse diversity, and mutations causing cognitive disorders reorganized synaptome maps. Synaptome technology and resources have wide-ranging application in studies of the normal and diseased brain.*NOTE: Fei Zhu, M{\'e}lissa Cizeron and Zhen Qiu contributed equally.",

author = "Fei Zhu and M{\'e}lissa Cizeron and Zhen Qiu and Ruth Benavides-Piccione and Kopanitsa, {Maksym V.} and Skene, {Nathan G.} and Babis Koniaris and Javier DeFelipe and Erik Frans{\'e}n and Komiyama, {Noboru H.} and Grant, {Seth G.N.}",

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AU - Zhu, Fei

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AU - Kopanitsa, Maksym V.

AU - Skene, Nathan G.

AU - Koniaris, Babis

AU - DeFelipe, Javier

AU - Fransén, Erik

AU - Komiyama, Noboru H.

AU - Grant, Seth G.N.

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AB - Synapses are found in vast numbers in the brain and contain complex proteomes. We developed genetic labeling and imaging methods to examine synaptic proteins in individual excitatory synapses across all regions of the mouse brain. Synapse catalogs were generated from the molecular and morphological features of a billion synapses. Each synapse subtype showed a unique anatomical distribution, and each brain region showed a distinct signature of synapse subtypes. Whole-brain synaptome cartography revealed spatial architecture from dendritic to global systems levels and previously unknown anatomical features. Synaptome mapping of circuits showed correspondence between synapse diversity and structural and functional connectomes. Behaviorally relevant patterns of neuronal activity trigger spatiotemporal postsynaptic responses sensitive to the structure of synaptome maps. Areas controlling higher cognitive function contain the greatest synapse diversity, and mutations causing cognitive disorders reorganized synaptome maps. Synaptome technology and resources have wide-ranging application in studies of the normal and diseased brain.*NOTE: Fei Zhu, Mélissa Cizeron and Zhen Qiu contributed equally.

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JO - Neuron

JF - Neuron

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Architecture of the Mouse Brain Synaptome

Abstract

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Projects

Molecular synapse imaging technology and resources

Lab and animal colony management

Datasets

Synaptome data with individual synapse parameters, types and subtypes across the 5 coronal mouse brain section

Profiles

Seth Grant

Noboru Komiyama

Cite this