Arginine Methylation of Vasa Protein Is Conserved across Phyla

Yohei Kirino, Anastassios Vourekas, Namwoo Kim, Flavia de Lima Alves, Juri Rappsilber, Peter S. Klein, Thomas A. Jongens, Zissimos Mourelatos

Research output: Contribution to journalArticlepeer-review

Abstract

Recent studies have uncovered an unexpected relationship between factors that are essential for germline development in Drosophila melanogaster: the arginine protein methyltransferase 5 (dPRMT5/Csul/Dart5) and its cofactor Valois, methylate the Piwi family protein Aub, enabling it to bind Tudor. The RNA helicase Vasa is another essential protein in germline development. Here, we report that mouse (mouse Vasa homolog), Xenopus laevis, and D. melanogaster Vasa proteins contain both symmetrical and asymmetrical dimethylarginines. We find that dPRMT5 is required for the production of sDMAs of Vasa in vivo. Furthermore, we find that the mouse Vasa homolog associates with Tudor domain-containing proteins, Tdrd1 and Tdrd6, as well as the Piwi proteins, Mili and Miwi. Arginine methylation is thus emerging as a conserved and pivotal post-translational modification of proteins that is essential for germline development.

Original languageEnglish
Pages (from-to)8148-8154
Number of pages7
JournalJournal of Biological Chemistry
Volume285
Issue number11
Early online date15 Jan 2010
DOIs
Publication statusPublished - 12 Mar 2010

Keywords

  • Drosophila
  • MicroRNA
  • Protein Methylation
  • RNA-Binding Protein
  • Spermatogenesis
  • Piwi
  • Tudor protein
  • Vasa Helicase
  • Drosophilia Oogenesis
  • PiRNA

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