Associations between Season and Gametocyte Dynamics in Chronic Plasmodium falciparum Infections

Amal Gadalla, Petra Schneider, Thomas S. Churcher, Elkhansaa Nassir, A Abdel-Muhsin, Lisa Ranford-Cartwright, Sarah Reece, Hamza Babiker

Research output: Contribution to journalArticlepeer-review

Abstract

In a markedly seasonal malaria setting, the transition from the transmission-free dry season to the transmission season depends on the resurgence of the mosquito population following the start of annual rains. The sudden onset of malaria outbreaks at the start of the transmission season suggests that parasites persist during the dry season and respond to either the reappearance of vectors, or correlated events, by increasing the production of transmission stages. Here, we investigate whether Plasmodium falciparum gametocyte density and the correlation between gametocyte density and parasite density show seasonal variation in chronic (largely asymptomatic) carriers in eastern Sudan.
Materials and Methods
We recruited and treated 123 malaria patients in the transmission season 2001. We then followed them monthly during four distinct consecutive epidemiological seasons: transmission season 1, transmission-free season, pre-clinical period, and transmission season 2. In samples collected from 25 participants who fulfilled the selection criteria of the current analysis, we used quantitative PCR (qPCR) and RT-qPCR to quantify parasite and gametocyte densities, respectively.
Results and Discussion
We observed a significant increase in gametocyte density and a significantly steeper positive correlation between gametocyte density and total parasite density during the pre-clinical period compared to the preceding transmission-free season. However, there was no corresponding increase in the density or prevalence of total parasites or gametocyte prevalence.
Original languageEnglish
Article number11
Number of pages15
JournalPLoS ONE
DOIs
Publication statusPublished - 21 Nov 2016

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