Attenuated tubal and endometrial urocortin 1 and corticotropin-releasing hormone receptor expression in ectopic pregnancy

L E Borges, A W Horne, S E McDonald, J L V Shaw, P C Lourenco, F Petraglia, H O D Critchley

Research output: Contribution to journalArticlepeer-review

Abstract

Fallopian tube (FT) and endometrial urocortin 1 (Ucn1) and corticotropin-releasing hormone (CRH)-receptor (CRH-R1/CRH-R2) expression were examined using quantitative real-time polymerase chain reaction (RT-PCR) and immunohistochemistry in nonpregnant and pregnant women (intrauterine, IUP; ectopic pregnancy, EP). Tubal Ucn1 messenger RNA (mRNA) expression was higher in luteal compared to follicular phase (P <.01) and equivalent to follicular phase in FT from EP. Tubal CRH-R1/CRH-R2 mRNA was lower in luteal phase (P <.05) and in FT from EP compared to follicular phase (P <.01). Ucn1 mRNA was lower in endometrium from EP compared to IUP (P <.05). Corticotropin-releasing hormone-R1 mRNA was higher in endometrium from EP compared to viable IUP (P <.05). No differences were observed in CRH-R2 expression. Corticotropin-releasing hormone-R1 protein was primarily localized to epithelium of FT and endometrium. Quantitative analysis of tubal CRH-R1 protein expression reflected that seen at the mRNA level but endometrial expression was equivocal. The demonstration of attenuated tubal/endometrial Ucn1/CRH-R expression in EP further supports a role of the CRH-family in embryo implantation.
Original languageEnglish
Pages (from-to)261-8
Number of pages8
JournalReproductive Sciences
Volume18
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • Urocortin 1
  • corticotrophin releasing hormone receptor
  • Fallopian tube
  • Endometrium
  • Ectopic pregnancy

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