Attenuated tubal and endometrial urocortin 1 and corticotropin-releasing hormone receptor expression in ectopic pregnancy

L E Borges; A W Horne; S E McDonald; J L V Shaw; P C Lourenco; F Petraglia; H O D Critchley

doi:10.1177/1933719110385132

Attenuated tubal and endometrial urocortin 1 and corticotropin-releasing hormone receptor expression in ectopic pregnancy

L E Borges, A W Horne, S E McDonald, J L V Shaw, P C Lourenco, F Petraglia, H O D Critchley

Research output: Contribution to journal › Article › peer-review

Abstract

Fallopian tube (FT) and endometrial urocortin 1 (Ucn1) and corticotropin-releasing hormone (CRH)-receptor (CRH-R1/CRH-R2) expression were examined using quantitative real-time polymerase chain reaction (RT-PCR) and immunohistochemistry in nonpregnant and pregnant women (intrauterine, IUP; ectopic pregnancy, EP). Tubal Ucn1 messenger RNA (mRNA) expression was higher in luteal compared to follicular phase (P <.01) and equivalent to follicular phase in FT from EP. Tubal CRH-R1/CRH-R2 mRNA was lower in luteal phase (P <.05) and in FT from EP compared to follicular phase (P <.01). Ucn1 mRNA was lower in endometrium from EP compared to IUP (P <.05). Corticotropin-releasing hormone-R1 mRNA was higher in endometrium from EP compared to viable IUP (P <.05). No differences were observed in CRH-R2 expression. Corticotropin-releasing hormone-R1 protein was primarily localized to epithelium of FT and endometrium. Quantitative analysis of tubal CRH-R1 protein expression reflected that seen at the mRNA level but endometrial expression was equivocal. The demonstration of attenuated tubal/endometrial Ucn1/CRH-R expression in EP further supports a role of the CRH-family in embryo implantation.

Original language	English
Pages (from-to)	261-8
Number of pages	8
Journal	Reproductive Sciences
Volume	18
Issue number	3
DOIs	https://doi.org/10.1177/1933719110385132
Publication status	Published - 2011

Keywords / Materials (for Non-textual outputs)

Urocortin 1
corticotrophin releasing hormone receptor
Fallopian tube
Endometrium
Ectopic pregnancy

Access to Document

10.1177/1933719110385132

Attenuated tubal and endometrial urocortin 1 and corticotropin-releasing hormone receptor expression in ectopic pregnancyAccepted author manuscript, 1.21 MB

Cite this

@article{c814d71e02c442b0b150c527b4b0226b,

title = "Attenuated tubal and endometrial urocortin 1 and corticotropin-releasing hormone receptor expression in ectopic pregnancy",

abstract = "Fallopian tube (FT) and endometrial urocortin 1 (Ucn1) and corticotropin-releasing hormone (CRH)-receptor (CRH-R1/CRH-R2) expression were examined using quantitative real-time polymerase chain reaction (RT-PCR) and immunohistochemistry in nonpregnant and pregnant women (intrauterine, IUP; ectopic pregnancy, EP). Tubal Ucn1 messenger RNA (mRNA) expression was higher in luteal compared to follicular phase (P <.01) and equivalent to follicular phase in FT from EP. Tubal CRH-R1/CRH-R2 mRNA was lower in luteal phase (P <.05) and in FT from EP compared to follicular phase (P <.01). Ucn1 mRNA was lower in endometrium from EP compared to IUP (P <.05). Corticotropin-releasing hormone-R1 mRNA was higher in endometrium from EP compared to viable IUP (P <.05). No differences were observed in CRH-R2 expression. Corticotropin-releasing hormone-R1 protein was primarily localized to epithelium of FT and endometrium. Quantitative analysis of tubal CRH-R1 protein expression reflected that seen at the mRNA level but endometrial expression was equivocal. The demonstration of attenuated tubal/endometrial Ucn1/CRH-R expression in EP further supports a role of the CRH-family in embryo implantation.",

keywords = "Urocortin 1, corticotrophin releasing hormone receptor, Fallopian tube, Endometrium , Ectopic pregnancy",

author = "Borges, {L E} and Horne, {A W} and McDonald, {S E} and Shaw, {J L V} and Lourenco, {P C} and F Petraglia and Critchley, {H O D}",

note = " {\textcopyright} 2011 by Society fo Gynecologic Investigation",

year = "2011",

doi = "10.1177/1933719110385132",

language = "English",

volume = "18",

pages = "261--8",

journal = "Reproductive Sciences",

issn = "1933-7191",

publisher = "Springer Nature",

number = "3",

}

TY - JOUR

T1 - Attenuated tubal and endometrial urocortin 1 and corticotropin-releasing hormone receptor expression in ectopic pregnancy

AU - Borges, L E

AU - Horne, A W

AU - McDonald, S E

AU - Shaw, J L V

AU - Lourenco, P C

AU - Petraglia, F

AU - Critchley, H O D

PY - 2011

Y1 - 2011

N2 - Fallopian tube (FT) and endometrial urocortin 1 (Ucn1) and corticotropin-releasing hormone (CRH)-receptor (CRH-R1/CRH-R2) expression were examined using quantitative real-time polymerase chain reaction (RT-PCR) and immunohistochemistry in nonpregnant and pregnant women (intrauterine, IUP; ectopic pregnancy, EP). Tubal Ucn1 messenger RNA (mRNA) expression was higher in luteal compared to follicular phase (P <.01) and equivalent to follicular phase in FT from EP. Tubal CRH-R1/CRH-R2 mRNA was lower in luteal phase (P <.05) and in FT from EP compared to follicular phase (P <.01). Ucn1 mRNA was lower in endometrium from EP compared to IUP (P <.05). Corticotropin-releasing hormone-R1 mRNA was higher in endometrium from EP compared to viable IUP (P <.05). No differences were observed in CRH-R2 expression. Corticotropin-releasing hormone-R1 protein was primarily localized to epithelium of FT and endometrium. Quantitative analysis of tubal CRH-R1 protein expression reflected that seen at the mRNA level but endometrial expression was equivocal. The demonstration of attenuated tubal/endometrial Ucn1/CRH-R expression in EP further supports a role of the CRH-family in embryo implantation.

AB - Fallopian tube (FT) and endometrial urocortin 1 (Ucn1) and corticotropin-releasing hormone (CRH)-receptor (CRH-R1/CRH-R2) expression were examined using quantitative real-time polymerase chain reaction (RT-PCR) and immunohistochemistry in nonpregnant and pregnant women (intrauterine, IUP; ectopic pregnancy, EP). Tubal Ucn1 messenger RNA (mRNA) expression was higher in luteal compared to follicular phase (P <.01) and equivalent to follicular phase in FT from EP. Tubal CRH-R1/CRH-R2 mRNA was lower in luteal phase (P <.05) and in FT from EP compared to follicular phase (P <.01). Ucn1 mRNA was lower in endometrium from EP compared to IUP (P <.05). Corticotropin-releasing hormone-R1 mRNA was higher in endometrium from EP compared to viable IUP (P <.05). No differences were observed in CRH-R2 expression. Corticotropin-releasing hormone-R1 protein was primarily localized to epithelium of FT and endometrium. Quantitative analysis of tubal CRH-R1 protein expression reflected that seen at the mRNA level but endometrial expression was equivocal. The demonstration of attenuated tubal/endometrial Ucn1/CRH-R expression in EP further supports a role of the CRH-family in embryo implantation.

KW - Urocortin 1

KW - corticotrophin releasing hormone receptor

KW - Fallopian tube

KW - Endometrium

KW - Ectopic pregnancy

UR - http://www.scopus.com/inward/record.url?scp=79952521986&partnerID=8YFLogxK

U2 - 10.1177/1933719110385132

DO - 10.1177/1933719110385132

M3 - Article

C2 - 20978183

SN - 1933-7191

VL - 18

SP - 261

EP - 268

JO - Reproductive Sciences

JF - Reproductive Sciences

IS - 3

ER -

Attenuated tubal and endometrial urocortin 1 and corticotropin-releasing hormone receptor expression in ectopic pregnancy

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Aberrant prokineticin expression as a ..

Cite this

Attenuated tubal and endometrial urocortin 1 and corticotropin-releasing hormone receptor expression in ectopic pregnancy

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Projects

Aberrant prokineticin expression as a ..

Cite this