Aurora B functions at the apical surface after specialized cytokinesis during morphogenesis in C. elegans

Xiaofei Bai, Michael Melesse, Christopher G Sorensen Turpin, Dillon E Sloan, Chin-Yi Chen, Wen-Cheng Wang, Po-Yi Lee, James R Simmons, Benjamin Nebenfuehr, Diana Mitchell, Lindsey R Klebanow, Nicholas Mattson, Eric Betzig, Bi-Chang Chen, Dhanya Cheerambathur, Joshua N Bembenek

Research output: Contribution to journalArticlepeer-review

Abstract

Although cytokinesis has been intensely studied, the way it is executed during development is not well understood, despite a long-standing appreciation that various aspects of cytokinesis vary across cell and tissue types. To address this, we investigated cytokinesis during the invariant Caenorhabditis elegans embryonic divisions and found several parameters that are altered at different stages in a reproducible manner. During early divisions, furrow ingression asymmetry and midbody inheritance is consistent, suggesting specific regulation of these events. During morphogenesis, we found several unexpected alterations to cytokinesis, including apical midbody migration in polarizing epithelial cells of the gut, pharynx and sensory neurons. Aurora B kinase, which is essential for several aspects of cytokinesis, remains apically localized in each of these tissues after internalization of midbody ring components. Aurora B inactivation disrupts cytokinesis and causes defects in apical structures, even if inactivated post-mitotically. Therefore, we demonstrate that cytokinesis is implemented in a specialized way during epithelial polarization and that Aurora B has a role in the formation of the apical surface.

Original languageEnglish
Article numberdev181099
JournalDevelopment
Volume147
Issue number1
DOIs
Publication statusPublished - 8 Jan 2020

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