Aurothiolates enhance the replication of Semliki Forest virus in the CNS and the exocrine pancreas

M F Scallan, J K Fazakerley

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The A7(74) strain of Semliki Forest virus (SFV) is avirulent and the L10 strain virulent in adult mice. A7(74) infection of adult mouse brain gives rise to small discrete foci of infection which, in immunocompetent animals, are cleared within 10 days. In contrast L10 infection results in a widespread and fatal central nervous system infection. Aurothiolates are linear, 2-coordinate complexes in which two ligands are covalently bound on either side of a gold nucleus in a +1 oxidation state (gold (I)). Pretreatment of A7(74) infected mice with two distinct aurothiolates (sodium aurothiomalate and aurothioglucose) resulted in significantly increased brain virus titers, and large confluent areas of infection in the brain similar to the pattern of infection seen with the L10 strain. The gold (I) moiety of aurothiolates was demonstrated to be the active component, since thiomalic acid when administered alone had no potentiating effect on the infection. Although both aurothiolates allowed productive replication and spread of A7(74) within the nature mouse brain, enhanced neuronal destruction was not apparent. There were no significant changes in virus distribution in any other tissue except for the exocrine pancreas and the myocardium where widespread infection of the acinar cells and occasional infected myocytes were observed.
Original languageEnglish
Pages (from-to)392-400
Number of pages9
JournalJournal of NeuroVirology
Issue number4
Publication statusPublished - Aug 1999

Keywords / Materials (for Non-textual outputs)

  • Age Factors
  • Animals
  • Aurothioglucose
  • Autoradiography
  • Brain
  • Cell Line
  • Female
  • Gold Sodium Thiomalate
  • Heart
  • Immunohistochemistry
  • Mice
  • Mice, Inbred BALB C
  • Pancreas
  • RNA, Viral
  • Semliki forest virus
  • Specific Pathogen-Free Organisms
  • Viral Plaque Assay
  • Virulence
  • Virus Replication


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