Auto-fluorescent PAMAM-based dendritic molecules and their potential application in pharmaceutical sciences

Alaa M.m. El-betany, Elbadawy A. Kamoun, Craig James, Abdulhakim Jangher, Ghaith Aljayyoussi, Peter Griffiths, Neil B. Mckeown, Mark Gumbleton

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The epithelial permeation of water-soluble fluorescent PAMAM dendrons based on 7H-benz[de] benzimidazo [2,1–a] isoquinoline-7-one as a fluorescent core across epithelial cell models MDCK I and MDCK II has been quantified.

Hydrodynamic radii have been derived from self-diffusion coefficients obtained via pulsed-gradient spin-echo Nuclear Magnetic Resonance (PGSE-NMR). Results indicate that these dendritic molecules are molecularly disperse, non-aggregating, and only slightly larger than their parent homologues. MDCK I permeability studies across epithelial barriers show that these dendritic molecules are biocompatible with the chosen epithelial in-vitro model and can permeate across MDCK cell monolayers. Permeability is demonstrated to be a property of dendritic size and cell barrier restrictiveness indicating that paracellular mechanisms play the predominant role in the transport of these molecules.
Original languageEnglish
Pages (from-to)119187
JournalInternational Journal of Pharmaceutics
Early online date2 Mar 2020
DOIs
Publication statusE-pub ahead of print - 2 Mar 2020

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