Automated image analysis for high-throughput quantitative detection of ER and PR expression levels in large-scale clinical studies: the TEAM Trial Experience

Dana Faratian; Charlene Kay; Tammy Robson; Fiona M Campbell; Margaret Grant; Dan Rea; John M S Bartlett

doi:10.1111/j.1365-2559.2009.03419.x

Automated image analysis for high-throughput quantitative detection of ER and PR expression levels in large-scale clinical studies: the TEAM Trial Experience

Dana Faratian, Charlene Kay, Tammy Robson, Fiona M Campbell, Margaret Grant, Dan Rea, John M S Bartlett

Deanery of Molecular, Genetic and Population Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Routine immunohistochemistry is regarded as a semiquantitative method for the evaluation of in situ protein expression. Analysis of tissue biomarkers in large clinical trials is central to the development of novel targeted approaches to therapy, requires the analysis of tens of thousands of data points, and frequently makes use of high-throughput analysis of tissue microarrays (TMAs). The aim of this study was to investigate the potential of image analysis for accurate and reproducible quantitative evaluation of biomarkers.

Original language	English
Pages (from-to)	587-93
Number of pages	7
Journal	Histopathology
Volume	55
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2559.2009.03419.x
Publication status	Published - Nov 2009

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10.1111/j.1365-2559.2009.03419.x

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title = "Automated image analysis for high-throughput quantitative detection of ER and PR expression levels in large-scale clinical studies: the TEAM Trial Experience",

abstract = "Routine immunohistochemistry is regarded as a semiquantitative method for the evaluation of in situ protein expression. Analysis of tissue biomarkers in large clinical trials is central to the development of novel targeted approaches to therapy, requires the analysis of tens of thousands of data points, and frequently makes use of high-throughput analysis of tissue microarrays (TMAs). The aim of this study was to investigate the potential of image analysis for accurate and reproducible quantitative evaluation of biomarkers.",

author = "Dana Faratian and Charlene Kay and Tammy Robson and Campbell, {Fiona M} and Margaret Grant and Dan Rea and Bartlett, {John M S}",

year = "2009",

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AU - Kay, Charlene

AU - Robson, Tammy

AU - Campbell, Fiona M

AU - Grant, Margaret

AU - Rea, Dan

AU - Bartlett, John M S

PY - 2009/11

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AB - Routine immunohistochemistry is regarded as a semiquantitative method for the evaluation of in situ protein expression. Analysis of tissue biomarkers in large clinical trials is central to the development of novel targeted approaches to therapy, requires the analysis of tens of thousands of data points, and frequently makes use of high-throughput analysis of tissue microarrays (TMAs). The aim of this study was to investigate the potential of image analysis for accurate and reproducible quantitative evaluation of biomarkers.

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DO - 10.1111/j.1365-2559.2009.03419.x

M3 - Article

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SN - 0309-0167

VL - 55

SP - 587

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JO - Histopathology

JF - Histopathology

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ER -

Automated image analysis for high-throughput quantitative detection of ER and PR expression levels in large-scale clinical studies: the TEAM Trial Experience

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