Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans

Maria Doitsidou, Nuria Flames, Albert C Lee, Alexander Boyanov, Oliver Hobert

Research output: Contribution to journalArticlepeer-review

Abstract

We describe an automated method to isolate mutant Caenorhabditis elegans that do not appropriately execute cellular differentiation programs. We used a fluorescence-activated sorting mechanism implemented in the COPAS Biosort machine to isolate mutants with subtle alterations in the cellular specificity of GFP expression. This methodology is considerably more efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons do not differentiate appropriately.

Original languageEnglish
Pages (from-to)869-72
Number of pages4
JournalNature Methods
Volume5
Issue number10
DOIs
Publication statusPublished - Oct 2008

Keywords

  • Animals
  • Caenorhabditis elegans
  • Dopamine
  • Flow Cytometry
  • Fluorescence
  • Green Fluorescent Proteins
  • Mutation
  • Neurons

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