B Cell Intrinsic MyD88 Signals Drive IFN-gamma Production from T Cells and Control Switching to IgG2c

Tom A. Barr, Sheila Brown, Pietro Mastroeni, David Gray

Research output: Contribution to journalArticlepeer-review

Abstract

The question of whether Ab responses to T-dependent Ags require B cell intrinsic signaling via the main TLR adaptor (MyD88) has become embroiled in confusion. In part this may be related to the methods used to analyze B cell intrinsic signaling. We have used a mixed bone marrow chimera model to generate mice in which the B cell compartment is completely deficient in MyD88 expression, while the other hematopoietic lineages are largely normal. These mice were immunized with T-dependent Ags or infected with Salmonella. We found that the Ag-specific IgG2c primary response was absolutely dependent on MyD88 signaling to B cells, while other Ig classes were not (IgG1 and IgG3) or much less so (IgG2b, IgA). The MyD88B(-/-) chimeric mice exhibited an impairment of development of IFN-gamma efector T cells, a likely contributory factor in the lack of IgG2c. We also found that B cell intrinsic MyD88 signals are required for the production of natural Abs. The data emphasize the nonredundant role of B cells as programmers of T cell differentiation in vivo.

Original languageEnglish
Pages (from-to)1005-1012
Number of pages8
JournalJournal of Immunology
Volume183
Issue number2
DOIs
Publication statusPublished - 15 Jul 2009

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