Bacterial infection disrupts clock gene expression to attenuate immune responses

María José De Leone, C. Esteban Hernando, Andrés Romanowski, Daniel A. Careno, Ana Faigón Soverna, Hequan Sun, Nicolás G. Bologna, Martín Vázquez, Korbinian Schneeberger, Marcelo J. Yanovsky

Research output: Contribution to journalArticlepeer-review

Abstract

The circadian clock modulates immune responses in plants and animals; however, it is unclear how host-pathogen interactions affect the clock. Here we analyzed clock function in Arabidopsis thaliana mutants with defective immune responses andfound that enhanced disease susceptibility 4 (eds4)displays alterations in several circadian rhythms.Mapping by sequencing revealed that EDS4 encodes the ortholog of NUCLEOPORIN 205, a corecomponent of the inner ring of the nuclear pore complex (NPC). Consistent with the idea that the NPCspecifically modulates clock function, we found astrong enrichment in core clock genes, as well as an increased nuclear to total mRNA accumulation,among genes that were differentially expressed ineds4 mutants. Interestingly, infection with Pseudomonas syringae in wild-type (WT) plants downregulated the expression of several morning core clockgenes as early as 1 h post-infection, including all members of the NIGHT LIGHT-INDUCIBLE ANDCLOCK-REGULATED (LNK) gene family, and thiseffect was attenuated in eds4. Furthermore, lnkmutants were more susceptible than the WT toP. syringae infection. These results indicate thatbacterial infection, acting in part through the NPC,alters core clock gene expression and/or mRNAaccumulation in a way that favors bacterial growthand disease susceptibility
Original languageEnglish
JournalCurrent Biology
DOIs
Publication statusPublished - 26 Mar 2020

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