We investigate the properties of a simple DNA-based nanodevice capable of detecting single nucleotide polymorphisms (SNPs) in unlabelled nucleic acid target sequences. Detection is achieved by a two-stage bait-and-switch process combining complementary-base hybridization and switching as molecular recognition criteria. A probe molecule is constructed from a single DNA strand designed to adopt a partial cruciform structure with a pair of exposed (unhybridized) strands. Upon target binding, a switchable cloverleaf construct (similar to a Holliday junction) is formed where the states are the open and closed junction conformations. Switching between these occurs by junction folding in the presence of divalent ions. A combination of steady-state and time-resolved fluorescence spectroscopy is used to measure Forster resonance energy transfer.
|Title of host publication||2009 SYMPOSIUM ON PHOTONICS AND OPTOELECTRONICS (SOPO 2009)|
|Place of Publication||NEW YORK|
|Publisher||Institute of Electrical and Electronics Engineers (IEEE)|
|Number of pages||6|
|Publication status||Published - 2009|
- single nucleotide polymorphisms
- fluorescence lifetime