BARX2 induces cadherin 6 expression and is a functional suppressor of ovarian cancer progression

G C Sellar, L Li, K P Watt, B D Nelkin, G J Rabiasz, E A Stronach, E P Miller, D J Porteous, J F Smyth, H Gabra

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The human homeobox BARX2 is located at 11q24-q25, within a minimal region associated with frequent loss of heterozygosity and adverse survival in epithelial ovarian cancer. BARX2 is a transcription factor that regulates transcription of specific cell adhesion molecules in the mouse. We show that BARX2 and cadherin 6 are expressed in normal human ovarian surface epithelium. BARX2 and cadherin 6 both have significantly lower expression in a clinical sample of endometrioid and clear cell ovarian cancers, as compared with serous or mixed mesodermal tumors. In a series of ovarian cancer cell lines, BARX2 expression showed a significant direct correlation with cadherin 6 expression. In OAW42, an ovarian cancer cell line that does not endogenously express BARX2, in vitro transfection of human BARX2 cDNA induced cadherin 6 expression. Transfection of BARX2 into OAW42 inhibited Matrigel invasion, haptotactic cellular migration to a collagen IV signal, and adhesion to collagen IV-coated plates. Our data demonstrate that BARX2 is expressed in the ovarian surface epithelium and has functional suppressor properties in ovarian cancer cells.
Original languageEnglish
Pages (from-to)6977-81
Number of pages5
JournalCancer Research
Issue number19
Publication statusPublished - 2001

Keywords / Materials (for Non-textual outputs)

  • Adenocarcinoma, Clear Cell
  • Cadherins
  • Carcinoma, Endometrioid
  • Cell Adhesion
  • Cell Movement
  • Collagen
  • Epithelium
  • Female
  • Gene Expression Regulation, Neoplastic
  • Homeodomain Proteins
  • Humans
  • Neoplasm Invasiveness
  • Ovarian Neoplasms
  • Reverse Transcriptase Polymerase Chain Reaction
  • S Phase
  • Transfection
  • Tumor Cells, Cultured


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