Abstract / Description of output
The human homeobox BARX2 is located at 11q24-q25, within a minimal region associated with frequent loss of heterozygosity and adverse survival in epithelial ovarian cancer. BARX2 is a transcription factor that regulates transcription of specific cell adhesion molecules in the mouse. We show that BARX2 and cadherin 6 are expressed in normal human ovarian surface epithelium. BARX2 and cadherin 6 both have significantly lower expression in a clinical sample of endometrioid and clear cell ovarian cancers, as compared with serous or mixed mesodermal tumors. In a series of ovarian cancer cell lines, BARX2 expression showed a significant direct correlation with cadherin 6 expression. In OAW42, an ovarian cancer cell line that does not endogenously express BARX2, in vitro transfection of human BARX2 cDNA induced cadherin 6 expression. Transfection of BARX2 into OAW42 inhibited Matrigel invasion, haptotactic cellular migration to a collagen IV signal, and adhesion to collagen IV-coated plates. Our data demonstrate that BARX2 is expressed in the ovarian surface epithelium and has functional suppressor properties in ovarian cancer cells.
Original language | English |
---|---|
Pages (from-to) | 6977-81 |
Number of pages | 5 |
Journal | Cancer Research |
Volume | 61 |
Issue number | 19 |
Publication status | Published - 2001 |
Keywords / Materials (for Non-textual outputs)
- Adenocarcinoma, Clear Cell
- Cadherins
- Carcinoma, Endometrioid
- Cell Adhesion
- Cell Movement
- Collagen
- Epithelium
- Female
- Gene Expression Regulation, Neoplastic
- Homeodomain Proteins
- Humans
- Neoplasm Invasiveness
- Ovarian Neoplasms
- Reverse Transcriptase Polymerase Chain Reaction
- S Phase
- Transfection
- Tumor Cells, Cultured