Base pair mismatch identification with DNA nanoswitch and long lifetime acridine fluorophore

Colin D. McGuinness; David Keszenman-Pereyra; Paul Dickinson; Colin J. Campbell; Beatrice A. Maltman; Till T. Bachmann; Peter Ghazal; Jason Crain

doi:10.1016/j.snb.2010.04.047

Base pair mismatch identification with DNA nanoswitch and long lifetime acridine fluorophore

Colin D. McGuinness, David Keszenman-Pereyra, Paul Dickinson, Colin J. Campbell, Beatrice A. Maltman, Till T. Bachmann, Peter Ghazal, Jason Crain

Research output: Contribution to journal › Article › peer-review

Abstract

Detection of single base pair mutations in an unlabelled nucleic acid target sequence is demonstrated using the time-resolved measurement of the Forster Resonance Energy Transfer (FRET) between a long lifetime acridine-based fluorophore and a non-fluorescent quencher molecule covalently bound to a DNA Holliday junction-based nanoswitch. (C) 2010 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	342-346
Number of pages	5
Journal	Sensors and Actuators B: Chemical
Volume	148
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2010.04.047
Publication status	Published - 30 Jun 2010

Keywords / Materials (for Non-textual outputs)

Fluorescence
Single photon counting
FRET
DNA
Holliday junction
FLUORESCENCE LIFETIME
9-AMINOACRIDINES
ASSAY

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10.1016/j.snb.2010.04.047

http://www.sciencedirect.com/science/article/pii/S0925400510003916

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title = "Base pair mismatch identification with DNA nanoswitch and long lifetime acridine fluorophore",

abstract = "Detection of single base pair mutations in an unlabelled nucleic acid target sequence is demonstrated using the time-resolved measurement of the Forster Resonance Energy Transfer (FRET) between a long lifetime acridine-based fluorophore and a non-fluorescent quencher molecule covalently bound to a DNA Holliday junction-based nanoswitch. (C) 2010 Elsevier B.V. All rights reserved.",

keywords = "Fluorescence, Single photon counting, FRET, DNA, Holliday junction, FLUORESCENCE LIFETIME, 9-AMINOACRIDINES, ASSAY",

author = "McGuinness, {Colin D.} and David Keszenman-Pereyra and Paul Dickinson and Campbell, {Colin J.} and Maltman, {Beatrice A.} and Bachmann, {Till T.} and Peter Ghazal and Jason Crain",

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doi = "10.1016/j.snb.2010.04.047",

language = "English",

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T1 - Base pair mismatch identification with DNA nanoswitch and long lifetime acridine fluorophore

AU - McGuinness, Colin D.

AU - Keszenman-Pereyra, David

AU - Dickinson, Paul

AU - Campbell, Colin J.

AU - Maltman, Beatrice A.

AU - Bachmann, Till T.

AU - Ghazal, Peter

AU - Crain, Jason

PY - 2010/6/30

Y1 - 2010/6/30

N2 - Detection of single base pair mutations in an unlabelled nucleic acid target sequence is demonstrated using the time-resolved measurement of the Forster Resonance Energy Transfer (FRET) between a long lifetime acridine-based fluorophore and a non-fluorescent quencher molecule covalently bound to a DNA Holliday junction-based nanoswitch. (C) 2010 Elsevier B.V. All rights reserved.

AB - Detection of single base pair mutations in an unlabelled nucleic acid target sequence is demonstrated using the time-resolved measurement of the Forster Resonance Energy Transfer (FRET) between a long lifetime acridine-based fluorophore and a non-fluorescent quencher molecule covalently bound to a DNA Holliday junction-based nanoswitch. (C) 2010 Elsevier B.V. All rights reserved.

KW - Fluorescence

KW - Single photon counting

KW - FRET

KW - DNA

KW - Holliday junction

KW - FLUORESCENCE LIFETIME

KW - 9-AMINOACRIDINES

KW - ASSAY

UR - http://www.scopus.com/inward/record.url?scp=77954083477&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2010.04.047

DO - 10.1016/j.snb.2010.04.047

M3 - Article

SN - 0925-4005

VL - 148

SP - 342

EP - 346

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

IS - 1

ER -

Base pair mismatch identification with DNA nanoswitch and long lifetime acridine fluorophore

Abstract

Keywords / Materials (for Non-textual outputs)

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