Projects per year
Optical biosensing based on the activation of fluorescent reporters offers a powerful methodology for the real-time molecular interrogation of pathology. Here we report a first-in-class, bimodal fluorescent reporter strategy for the simultaneous and highly specific detection of two independent proteases (thrombin and matrix metalloproteases (MMPs)) pivotal in the fibroproliferative process surrounding lung cancer, based on a dual, multiplexing, peptide FRET system. This sophisticated synthetic smartprobe, with a molecular weight of 6 kDa, contains two independent fluorophores and quenchers that generate photonic signatures at two specific wavelengths upon activation by target enzymes within human lung cancer tissue.
|Journal||Organic & Biomolecular chemistry|
|Early online date||23 Aug 2018|
|Publication status||E-pub ahead of print - 23 Aug 2018|
FingerprintDive into the research topics of 'Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer'. Together they form a unique fingerprint.
- 2 Finished
Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging
1/10/13 → 31/03/19
Molecular Optical Imaging of Key Targets in the Fibrogenic Pathway in Man
Bradley, M., Dhaliwal, K., Haslett, C., Hirani, N. & McGorum, B.
1/09/12 → 31/12/15
Dataset for "BIMODAL FLUOROGENIC SENSING OF MATRIX PROTEOLYTIC SIGNATURES"
Megia-Fernandez, A. (Creator), Edinburgh DataShare, 28 Sep 2018