Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer

Ross Stevenson; H. C. Baxter; A. Aitken; T. Brown; R. L. Baxter

doi:10.1016/j.bioorg.2007.12.005

Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer

Ross Stevenson, H. C. Baxter, A. Aitken, T. Brown, R. L. Baxter

Research output: Contribution to journal › Article › peer-review

Abstract

A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3 gamma. A single aptamer, which showed similar affinities (K-D similar to 10(-8) M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.

Original language	English
Pages (from-to)	215-219
Number of pages	5
Journal	Bioorganic Chemistry
Volume	36
Issue number	5
DOIs	https://doi.org/10.1016/j.bioorg.2007.12.005
Publication status	Published - Oct 2008

Keywords

Aptamer
14-3-3
TSE
CJD
Affinity
Protein–DNA interaction

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10.1016/j.bioorg.2007.12.005

http://www.sciencedirect.com/science/article/pii/S0045206807000909

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title = "Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer",

abstract = "A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3 gamma. A single aptamer, which showed similar affinities (K-D similar to 10(-8) M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep. ",

keywords = "Aptamer , 14-3-3, TSE, CJD, Affinity, Protein–DNA interaction",

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AU - Aitken, A.

AU - Brown, T.

AU - Baxter, R. L.

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AB - A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3 gamma. A single aptamer, which showed similar affinities (K-D similar to 10(-8) M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.

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KW - 14-3-3

KW - TSE

KW - CJD

KW - Affinity

KW - Protein–DNA interaction

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JO - Bioorganic Chemistry

JF - Bioorganic Chemistry

SN - 0045-2068

IS - 5

ER -

Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer

Abstract

Keywords

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