Binding of transcription factors and creation of a large nucleoprotein complex on the human cytomegalovirus enhancer

P Ghazal, H Lubon, B Fleckenstein, L Hennighausen

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of the human cytomegalovirus immediate early region 1 enhancer on transcription was studied in vitro with HeLa cell nuclear extract. Stimulation of in vitro transcription mediated by the enhancer element involves its recognition by specific trans-acting factors present in the nuclear extract. DNase I protection analysis was used to determine at the nucleotide level those enhancer sequences that interact with nuclear factors. At least nine sites of protein-DNA interaction were detected over approximately 400 base pairs of enhancer sequence. The regions of nuclease protection are associated with 21-, 19-, 18-, and 17-base-pair repeat elements as well as with a unique sequence, creating a large nucleoprotein complex. The relationship between the protein binding and the activity of the immediate early region 1 enhancer is discussed.
Original languageEnglish
Pages (from-to)3658-62
Number of pages5
JournalProceedings of the National Academy of Sciences
Volume84
Issue number11
DOIs
Publication statusPublished - 1987

Keywords

  • Base Sequence
  • Cell Nucleus
  • Cytomegalovirus
  • Enhancer Elements, Genetic
  • Genes
  • Genes, Regulator
  • Genes, Viral
  • HeLa Cells
  • Humans
  • Plasmids
  • Promoter Regions, Genetic
  • Templates, Genetic
  • Transcription Factors
  • Transcription, Genetic

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