Biogenesis of Y RNA-derived small RNAs is independent of the microRNA pathway

Francisco Esteban Nicolas, Adam E Hall, Tibor Csorba, Carly Turnbull, Tamas Dalmay

Research output: Contribution to journalArticlepeer-review

Abstract

Y RNAs are approximately 100 nucleotide long conserved cytoplasmic non-coding RNAs, which produce smaller RNA fragments during apoptosis. Here we show that these smaller RNA molecules are also produced in non-stressed cells and in a range of human cancerous and non-cancerous cell types. Recent reports have speculated that the cleavage products of Y RNAs enter the microRNA pathway. We tested this hypothesis and found that Y5 and Y3 RNA fragments are Dicer independent, they are in different complexes than microRNAs and that they are not co-immunoprecipitated with Ago2. Therefore we conclude that Y RNA fragments do not enter the microRNA pathway.

Original languageEnglish
Pages (from-to)1226-30
Number of pages5
JournalFEBS Letters
Volume586
Issue number8
DOIs
Publication statusPublished - 24 Apr 2012

Keywords

  • Argonaute Proteins
  • Cytoplasm
  • DEAD-box RNA Helicases
  • HCT116 Cells
  • Humans
  • Membrane Proteins
  • MicroRNAs
  • RNA, Untranslated
  • Ribonuclease III
  • Signal Transduction
  • Tumor Cells, Cultured

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