BIOLOGICAL PRODUCTION OF SEMISYNTHETIC OPIATES USING GENETICALLY-ENGINEERED BACTERIA

C E  FRENCH; A M  HAILES; D A  RATHBONE; M T  LONG; D L  WILLEY; N C  BRUCE; Chris French

BIOLOGICAL PRODUCTION OF SEMISYNTHETIC OPIATES USING GENETICALLY-ENGINEERED BACTERIA

C E FRENCH, A M HAILES, D A RATHBONE, M T LONG, D L WILLEY, N C BRUCE, Chris French

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Semisynthetic derivatives of morphine and related alkaloids re in widespread clinical use. Due to the complexity of these molecules, however, chemical transformations are difficult to achieve in high yields. We recently identified the powerful analgesic hydromorphone as an intermediate in the metabolism of morphine by Pseudomonas putida M10. Here we describe the construction of recombinant strains of Escherichia coli that express morphine dehydrogenase and morphinone reductase. These strains are capable of efficiently transforming the naturally occurring alkaloids morphine and codeine to hydro-morphone and antitussive hydrocodone, respectively. Our results demonstrate the potential for recombinant DNA technology to provide biological routes for the synthesis of known and novel semi-synthetic opiate drugs.

Original language	English
Pages (from-to)	674-676
Number of pages	3
Journal	Bio-technology
Volume	13
Issue number	7
Publication status	Published - Jul 1995

Cite this

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title = "BIOLOGICAL PRODUCTION OF SEMISYNTHETIC OPIATES USING GENETICALLY-ENGINEERED BACTERIA",

abstract = "Semisynthetic derivatives of morphine and related alkaloids re in widespread clinical use. Due to the complexity of these molecules, however, chemical transformations are difficult to achieve in high yields. We recently identified the powerful analgesic hydromorphone as an intermediate in the metabolism of morphine by Pseudomonas putida M10. Here we describe the construction of recombinant strains of Escherichia coli that express morphine dehydrogenase and morphinone reductase. These strains are capable of efficiently transforming the naturally occurring alkaloids morphine and codeine to hydro-morphone and antitussive hydrocodone, respectively. Our results demonstrate the potential for recombinant DNA technology to provide biological routes for the synthesis of known and novel semi-synthetic opiate drugs.",

author = "FRENCH, {C E} and HAILES, {A M} and RATHBONE, {D A} and LONG, {M T} and WILLEY, {D L} and BRUCE, {N C} and Chris French",

year = "1995",

month = jul,

language = "English",

volume = "13",

pages = "674--676",

journal = "Bio-technology",

issn = "0733-222X",

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T1 - BIOLOGICAL PRODUCTION OF SEMISYNTHETIC OPIATES USING GENETICALLY-ENGINEERED BACTERIA

AU - FRENCH, C E

AU - HAILES, A M

AU - RATHBONE, D A

AU - LONG, M T

AU - WILLEY, D L

AU - BRUCE, N C

AU - French, Chris

PY - 1995/7

Y1 - 1995/7

N2 - Semisynthetic derivatives of morphine and related alkaloids re in widespread clinical use. Due to the complexity of these molecules, however, chemical transformations are difficult to achieve in high yields. We recently identified the powerful analgesic hydromorphone as an intermediate in the metabolism of morphine by Pseudomonas putida M10. Here we describe the construction of recombinant strains of Escherichia coli that express morphine dehydrogenase and morphinone reductase. These strains are capable of efficiently transforming the naturally occurring alkaloids morphine and codeine to hydro-morphone and antitussive hydrocodone, respectively. Our results demonstrate the potential for recombinant DNA technology to provide biological routes for the synthesis of known and novel semi-synthetic opiate drugs.

AB - Semisynthetic derivatives of morphine and related alkaloids re in widespread clinical use. Due to the complexity of these molecules, however, chemical transformations are difficult to achieve in high yields. We recently identified the powerful analgesic hydromorphone as an intermediate in the metabolism of morphine by Pseudomonas putida M10. Here we describe the construction of recombinant strains of Escherichia coli that express morphine dehydrogenase and morphinone reductase. These strains are capable of efficiently transforming the naturally occurring alkaloids morphine and codeine to hydro-morphone and antitussive hydrocodone, respectively. Our results demonstrate the potential for recombinant DNA technology to provide biological routes for the synthesis of known and novel semi-synthetic opiate drugs.

M3 - Article

SN - 0733-222X

VL - 13

SP - 674

EP - 676

JO - Bio-technology

JF - Bio-technology

IS - 7

ER -

BIOLOGICAL PRODUCTION OF SEMISYNTHETIC OPIATES USING GENETICALLY-ENGINEERED BACTERIA

Abstract

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