Bluetongue and Epizootic Haemorrhagic Disease virus in local breeds of cattle in Kenya

P G Toye; C A Batten; H Kiara; M R Henstock; L Edwards; S Thumbi; E J Poole; I G Handel; Mark Bronsvoort; O Hanotte; J A W Coetzer; M E J Woolhouse; C A L Oura

doi:10.1016/j.rvsc.2012.11.001

Bluetongue and Epizootic Haemorrhagic Disease virus in local breeds of cattle in Kenya

P G Toye, C A Batten, H Kiara, M R Henstock, L Edwards, S Thumbi, E J Poole, I G Handel, Mark Bronsvoort, O Hanotte, J A W Coetzer, M E J Woolhouse, C A L Oura

Research output: Contribution to journal › Article › peer-review

Abstract

The presence of bluetongue virus (BTV) and Epizootic Haemorrhagic Disease virus (EHDV) in indigenous calves in western Kenya was investigated. Serum was analysed for BTV and EHDV antibodies. The population seroprevalences for BTV and EHDV for calves at 51weeks of age were estimated to be 0.942 (95% CI 0.902-0.970) and 0.637 (95% CI 0.562-0.710), respectively, indicating high levels of circulating BTV and EHDV. The odds ratio of being positive for BTV if EHDV positive was estimated to be 2.57 (95% CI 1.37-4.76). When 99 calves were tested for BTV and EHDV RNA by real-time RT-PCR, 88.9% and 63.6% were positive, respectively. Comparison of the serology and real-time RT-PCR results revealed an unexpectedly large number of calves that were negative by serology but positive by real-time RT-PCR for EHDV. Eight samples positive for BTV RNA were serotyped using 24 serotype-specific real-time RT-PCR assays. Nine BTV serotypes were detected, indicating that the cattle were infected with a heterogeneous population of BTVs. The results show that BTV and EHDV are highly prevalent, with cattle being infected from an early age.

Original language	English
Pages (from-to)	769-773
Journal	Research in Veterinary Science
Volume	94
Early online date	20 Dec 2012
DOIs	https://doi.org/10.1016/j.rvsc.2012.11.001
Publication status	Published - 2013

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10.1016/j.rvsc.2012.11.001

Bluetongue and Epizootic Haemorrhagic Disease virus in local breeds of cattle in Kenya
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http://www.sciencedirect.com/science/article/pii/S0034528812003347

ISP1: Analysis and prediction in complex animal systems
Tenesa, A. (Principal Investigator), Archibald, A. (Co-investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Freeman, T. (Co-investigator), Haley, C. (Co-investigator), Hocking, P. (Co-investigator), Houston, R. (Co-investigator), Hume, D. (Co-investigator), Joshi, A. (Co-investigator), Law, A. (Co-investigator), Michoel, T. (Co-investigator), Summers, K. (Co-investigator), Vernimmen, D. (Co-investigator), Watson, M. (Co-investigator), Wiener, P. (Co-investigator), Wilson, A. (Co-investigator), Woolliams, J. (Co-investigator), Ait-Ali, T. (Researcher), Barnett, M. (Researcher), Carlisle, A. (Researcher), Finlayson, H. (Researcher), Haga, I. (Researcher), Karavolos, M. (Researcher), Matika, O. (Researcher), Paterson, T. (Researcher), Paton, B. (Researcher), Pong-Wong, R. (Researcher), Robert, C. (Researcher) & Robertson, G. (Researcher)
BBSRC
1/04/12 → 31/03/17
Project: Research
IDEAL: Infection dynamics and disease burden of East African cattle
Woolhouse, M. (Principal Investigator) & Bronsvoort, M. (Co-investigator)
UK-based charities
1/09/06 → 30/04/13
Project: Research

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Toye, P. G., Batten, C. A., Kiara, H., Henstock, M. R., Edwards, L., Thumbi, S., Poole, E. J., Handel, I. G., Bronsvoort, M., Hanotte, O., Coetzer, J. A. W., Woolhouse, M. E. J., & Oura, C. A. L. (2013). Bluetongue and Epizootic Haemorrhagic Disease virus in local breeds of cattle in Kenya. Research in Veterinary Science, 94, 769-773. https://doi.org/10.1016/j.rvsc.2012.11.001

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title = "Bluetongue and Epizootic Haemorrhagic Disease virus in local breeds of cattle in Kenya",

abstract = "The presence of bluetongue virus (BTV) and Epizootic Haemorrhagic Disease virus (EHDV) in indigenous calves in western Kenya was investigated. Serum was analysed for BTV and EHDV antibodies. The population seroprevalences for BTV and EHDV for calves at 51weeks of age were estimated to be 0.942 (95% CI 0.902-0.970) and 0.637 (95% CI 0.562-0.710), respectively, indicating high levels of circulating BTV and EHDV. The odds ratio of being positive for BTV if EHDV positive was estimated to be 2.57 (95% CI 1.37-4.76). When 99 calves were tested for BTV and EHDV RNA by real-time RT-PCR, 88.9% and 63.6% were positive, respectively. Comparison of the serology and real-time RT-PCR results revealed an unexpectedly large number of calves that were negative by serology but positive by real-time RT-PCR for EHDV. Eight samples positive for BTV RNA were serotyped using 24 serotype-specific real-time RT-PCR assays. Nine BTV serotypes were detected, indicating that the cattle were infected with a heterogeneous population of BTVs. The results show that BTV and EHDV are highly prevalent, with cattle being infected from an early age.",

author = "Toye, {P G} and Batten, {C A} and H Kiara and Henstock, {M R} and L Edwards and S Thumbi and Poole, {E J} and Handel, {I G} and Mark Bronsvoort and O Hanotte and Coetzer, {J A W} and Woolhouse, {M E J} and Oura, {C A L}",

note = "The IDEAL project was generously funded by the Wellcome Trust (Project 079445). The funders were not involved in the study design, in the collection, analysis and interpretation of the data, in the writing of the manuscript or in the decision to submit the manuscript for publication.",

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doi = "10.1016/j.rvsc.2012.11.001",

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AU - Toye, P G

AU - Batten, C A

AU - Kiara, H

AU - Henstock, M R

AU - Edwards, L

AU - Thumbi, S

AU - Poole, E J

AU - Handel, I G

AU - Bronsvoort, Mark

AU - Hanotte, O

AU - Coetzer, J A W

AU - Woolhouse, M E J

AU - Oura, C A L

N1 - The IDEAL project was generously funded by the Wellcome Trust (Project 079445). The funders were not involved in the study design, in the collection, analysis and interpretation of the data, in the writing of the manuscript or in the decision to submit the manuscript for publication.

PY - 2013

Y1 - 2013

N2 - The presence of bluetongue virus (BTV) and Epizootic Haemorrhagic Disease virus (EHDV) in indigenous calves in western Kenya was investigated. Serum was analysed for BTV and EHDV antibodies. The population seroprevalences for BTV and EHDV for calves at 51weeks of age were estimated to be 0.942 (95% CI 0.902-0.970) and 0.637 (95% CI 0.562-0.710), respectively, indicating high levels of circulating BTV and EHDV. The odds ratio of being positive for BTV if EHDV positive was estimated to be 2.57 (95% CI 1.37-4.76). When 99 calves were tested for BTV and EHDV RNA by real-time RT-PCR, 88.9% and 63.6% were positive, respectively. Comparison of the serology and real-time RT-PCR results revealed an unexpectedly large number of calves that were negative by serology but positive by real-time RT-PCR for EHDV. Eight samples positive for BTV RNA were serotyped using 24 serotype-specific real-time RT-PCR assays. Nine BTV serotypes were detected, indicating that the cattle were infected with a heterogeneous population of BTVs. The results show that BTV and EHDV are highly prevalent, with cattle being infected from an early age.

AB - The presence of bluetongue virus (BTV) and Epizootic Haemorrhagic Disease virus (EHDV) in indigenous calves in western Kenya was investigated. Serum was analysed for BTV and EHDV antibodies. The population seroprevalences for BTV and EHDV for calves at 51weeks of age were estimated to be 0.942 (95% CI 0.902-0.970) and 0.637 (95% CI 0.562-0.710), respectively, indicating high levels of circulating BTV and EHDV. The odds ratio of being positive for BTV if EHDV positive was estimated to be 2.57 (95% CI 1.37-4.76). When 99 calves were tested for BTV and EHDV RNA by real-time RT-PCR, 88.9% and 63.6% were positive, respectively. Comparison of the serology and real-time RT-PCR results revealed an unexpectedly large number of calves that were negative by serology but positive by real-time RT-PCR for EHDV. Eight samples positive for BTV RNA were serotyped using 24 serotype-specific real-time RT-PCR assays. Nine BTV serotypes were detected, indicating that the cattle were infected with a heterogeneous population of BTVs. The results show that BTV and EHDV are highly prevalent, with cattle being infected from an early age.

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DO - 10.1016/j.rvsc.2012.11.001

M3 - Article

C2 - 23261160

SN - 1532-2661

VL - 94

SP - 769

EP - 773

JO - Research in Veterinary Science

JF - Research in Veterinary Science

ER -

Bluetongue and Epizootic Haemorrhagic Disease virus in local breeds of cattle in Kenya

Abstract

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Projects

ISP1: Analysis and prediction in complex animal systems

IDEAL: Infection dynamics and disease burden of East African cattle

Cite this