BOLA CLASS-I CHARGE HETEROGENEITY REFLECTS THE EXPRESSION OF MORE THAN 2 LOCI

SWK ALMURRANI*, EJ GLASS, J HOPKINS

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Internationally recognized allo-antisera in lymphocyte microcytotoxicity assays are thought to detect allelic products of a single highly polymorphic class I locus. A recent report suggested that two bovine lymphocyte antigen (BoLA) class I loci are expressed at the protein level. However, 1D-IEF analysis of BoLA class I molecules reveals multi-band patterns which cannot be reconciled with the reported number of loci. The aim of this study was to investigate the origins of the charge diversity of BoLA class I molecules observed using 1D-IEF.

BoLA class I molecules appear to be glycosylated at a single N-linked position with a complex type carbohydrate moiety which has up to three terminal sialic acid residues. Class I molecules immunoprecipitated from resting bovine PBL are not phosphorylated. Neither modification is responsible for the observed charge heterogeneity.

Peptide mapping reveals that different BoLA charge variants have distinct digestion patterns. Furthermore, a number of different polypeptides are associated with each serological specificity. These polypeptides appear to be encoded by different loci which exist in linkage disequilibrium. The number of charge variants with different peptide maps indicates that the BoLA system has a minimum of three class I loci expressed at the protein level.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalAnimal Genetics
Volume25
Issue number3
Publication statusPublished - Jun 1994

Keywords / Materials (for Non-textual outputs)

  • BOLA
  • GLYCOSYLATION
  • PHOSPHORYLATION
  • PEPTIDE MAPPING
  • MAJOR HISTOCOMPATIBILITY COMPLEX
  • JOINT REPORT
  • ANTIGENS
  • GENES
  • CELLS
  • CDNA
  • ACTIVATION
  • HLA-A,B,C
  • PROTEINS

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