Bonds broken and formed during the mixed-linkage glucan : xyloglucan endotransglucosylase reaction catalysed by Equisetum hetero-trans-β-glucanase

Thomas J Simmons, Stephen Fry

Research output: Contribution to journal › Article › peer-review

Abstract

Equisetum hetero-trans-β-glucanase (HTG) preferentially catalyses hetero-transglycosylation, transferring a length of mixedlinkage β-glucan (MLG) polysaccharide to xyloglucan. We elucidated the MLG glycosyl sequence cleaved, and show HTG can attack MLG at any such sequence,irrespective of its distance from the termini.
Original languageEnglish
Pages (from-to)1055-1070
JournalBiochemical Journal
Volume474
Issue number7
Early online date20 Jan 2017
DOIs
Publication statusPublished - 8 Mar 2017

Keywords

  • mixed-linkage glucan
  • xyloglucan
  • transglycanases
  • hetero-transglycosylation
  • Equisetum
  • cell wall

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  • 1055.full

    2017 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).

    Final published version, 1.01 MBLicence: Creative Commons: Attribution (CC-BY)

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