Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION: CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION

Ceara S. Suther; Peter Wilson; J. Gautron; Ian Dunn; Maureen M. Bain

Abstract / Description of output

The cuticle, which forms the outermost protective layer of the egg, is produced during the terminal phase of shell formation but it is not known if it is produced in the shell gland or vagina. The aim of this study was to establish if Ovocalyxin-32 (OCX-32), a precursor protein of the eggshell cuticle is localized in the SGP or the Vagina when oviposition is endocrinologically manipulated. Immunocytochemistry for OCX- 32 was carried out on SGP and vagina tissues derived from laying hens injected with GnRH or AVT to induce premature oviposition. The % area of cells staining positive for OCX-32 was determined by analyzing 4 areas per tissue using ImageJ software. The cuticle quality and the colour of the eggs laid before and after treatment were also determined using an established method. AVT induced oviposition had a large and negative effect on cuticle and pigment deposition whereas there was little change following GnRH injection, despite both causing oviposition at the same time. The % area of ciliated surface epithelial cells staining positively for OCX-32 was higher in the AVT derived SGP tissues than for GnRH (22.7±1.7% v 16.0±1.8%; P=0.016, n=10/11). There was no specific staining for OCX-32 in the vagina from either treatment confirming that the cuticle is deposited in the shell gland pouch (uterus) and not the vagina.

Original language	English
Publication status	Published - 6 Sept 2017
Event	EGGMEAT 2017’ XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat - Duration: 6 Sept 2017 → 6 Sept 2017

Conference

Conference	EGGMEAT 2017’ XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat
Period	6/09/17 → 6/09/17

Access to Document

http://www.wpsa.com/index.php/wpsa-proceedings/2017/xxiii-european-symposium-on-the-quality-of-poultry-meat-and-xvii-european-symposium-on-the-quality-of-eggs-and-egg-products/3824-cuticle-deposition-occurs-in-the-shell-gland-uterus-and-not-the-vagina-during-the-terminal-phase-of-shell-formation/file

Fingerprint

Dive into the research topics of 'Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION: CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION'. Together they form a unique fingerprint.

ISP 2017/22-Dunn Ian
Dunn, I.
BBSRC
1/04/17 → 31/03/22
Project: Research
Cute-Egg, improvement of eggshell cuticle quality to reduce vertical transmission of zoonotic and pathogenic organisms
Dunn, I.
UK industry, commerce and public corporations
1/04/13 → 30/09/16
Project: Research
Control of development and reproductive traits
Burdon, T., Argyle, D., Ashworth, C., Beard, P., Brunton, P., Burt, D., Clinton, M., Dunn, I., Farquharson, C., Headon, D., Hocking, P., Hohenstein, P., Hume, D., Jackson, I., McColl, B., McGrew, M., McLachlan, G., Sang, H., Summers, K. & Whitelaw, B.
BBSRC
1/04/12 → 31/03/17
Project: Research

Cite this

Suther, C. S., Wilson, P., Gautron, J., Dunn, I., & Bain, M. M. (2017). Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION: CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION. Abstract from EGGMEAT 2017’ XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat. http://www.wpsa.com/index.php/wpsa-proceedings/2017/xxiii-european-symposium-on-the-quality-of-poultry-meat-and-xvii-european-symposium-on-the-quality-of-eggs-and-egg-products/3824-cuticle-deposition-occurs-in-the-shell-gland-uterus-and-not-the-vagina-during-the-terminal-phase-of-shell-formation/file

Suther, Ceara S. ; Wilson, Peter ; Gautron, J. et al. / Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION. Abstract from EGGMEAT 2017’ XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat.

@conference{2767ad55b5c1409d9c7e102c9c9aa645,

title = "Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION: CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION",

abstract = "The cuticle, which forms the outermost protective layer of the egg, is produced during the terminal phase of shell formation but it is not known if it is produced in the shell gland or vagina. The aim of this study was to establish if Ovocalyxin-32 (OCX-32), a precursor protein of the eggshell cuticle is localized in the SGP or the Vagina when oviposition is endocrinologically manipulated. Immunocytochemistry for OCX- 32 was carried out on SGP and vagina tissues derived from laying hens injected with GnRH or AVT to induce premature oviposition. The % area of cells staining positive for OCX-32 was determined by analyzing 4 areas per tissue using ImageJ software. The cuticle quality and the colour of the eggs laid before and after treatment were also determined using an established method. AVT induced oviposition had a large and negative effect on cuticle and pigment deposition whereas there was little change following GnRH injection, despite both causing oviposition at the same time. The % area of ciliated surface epithelial cells staining positively for OCX-32 was higher in the AVT derived SGP tissues than for GnRH (22.7±1.7% v 16.0±1.8%; P=0.016, n=10/11). There was no specific staining for OCX-32 in the vagina from either treatment confirming that the cuticle is deposited in the shell gland pouch (uterus) and not the vagina.",

author = "Suther, {Ceara S.} and Peter Wilson and J. Gautron and Ian Dunn and Bain, {Maureen M.}",

year = "2017",

month = sep,

day = "6",

language = "English",

note = "EGGMEAT 2017{\textquoteright} XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat ; Conference date: 06-09-2017 Through 06-09-2017",

}

Suther, CS, Wilson, P, Gautron, J, Dunn, I & Bain, MM 2017, 'Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION: CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION', EGGMEAT 2017’ XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat, 6/09/17 - 6/09/17. <http://www.wpsa.com/index.php/wpsa-proceedings/2017/xxiii-european-symposium-on-the-quality-of-poultry-meat-and-xvii-european-symposium-on-the-quality-of-eggs-and-egg-products/3824-cuticle-deposition-occurs-in-the-shell-gland-uterus-and-not-the-vagina-during-the-terminal-phase-of-shell-formation/file>

Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION: CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION. / Suther, Ceara S.; Wilson, Peter; Gautron, J. et al.
2017. Abstract from EGGMEAT 2017’ XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat.

Research output: Contribution to conference › Abstract › peer-review

TY - CONF

T1 - Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION

T2 - EGGMEAT 2017’ XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat

AU - Suther, Ceara S.

AU - Wilson, Peter

AU - Gautron, J.

AU - Dunn, Ian

AU - Bain, Maureen M.

PY - 2017/9/6

Y1 - 2017/9/6

N2 - The cuticle, which forms the outermost protective layer of the egg, is produced during the terminal phase of shell formation but it is not known if it is produced in the shell gland or vagina. The aim of this study was to establish if Ovocalyxin-32 (OCX-32), a precursor protein of the eggshell cuticle is localized in the SGP or the Vagina when oviposition is endocrinologically manipulated. Immunocytochemistry for OCX- 32 was carried out on SGP and vagina tissues derived from laying hens injected with GnRH or AVT to induce premature oviposition. The % area of cells staining positive for OCX-32 was determined by analyzing 4 areas per tissue using ImageJ software. The cuticle quality and the colour of the eggs laid before and after treatment were also determined using an established method. AVT induced oviposition had a large and negative effect on cuticle and pigment deposition whereas there was little change following GnRH injection, despite both causing oviposition at the same time. The % area of ciliated surface epithelial cells staining positively for OCX-32 was higher in the AVT derived SGP tissues than for GnRH (22.7±1.7% v 16.0±1.8%; P=0.016, n=10/11). There was no specific staining for OCX-32 in the vagina from either treatment confirming that the cuticle is deposited in the shell gland pouch (uterus) and not the vagina.

AB - The cuticle, which forms the outermost protective layer of the egg, is produced during the terminal phase of shell formation but it is not known if it is produced in the shell gland or vagina. The aim of this study was to establish if Ovocalyxin-32 (OCX-32), a precursor protein of the eggshell cuticle is localized in the SGP or the Vagina when oviposition is endocrinologically manipulated. Immunocytochemistry for OCX- 32 was carried out on SGP and vagina tissues derived from laying hens injected with GnRH or AVT to induce premature oviposition. The % area of cells staining positive for OCX-32 was determined by analyzing 4 areas per tissue using ImageJ software. The cuticle quality and the colour of the eggs laid before and after treatment were also determined using an established method. AVT induced oviposition had a large and negative effect on cuticle and pigment deposition whereas there was little change following GnRH injection, despite both causing oviposition at the same time. The % area of ciliated surface epithelial cells staining positively for OCX-32 was higher in the AVT derived SGP tissues than for GnRH (22.7±1.7% v 16.0±1.8%; P=0.016, n=10/11). There was no specific staining for OCX-32 in the vagina from either treatment confirming that the cuticle is deposited in the shell gland pouch (uterus) and not the vagina.

M3 - Abstract

Y2 - 6 September 2017 through 6 September 2017

ER -

Suther CS, Wilson P, Gautron J, Dunn I, Bain MM. Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION: CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION. 2017. Abstract from EGGMEAT 2017’ XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat.

Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION: CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION

Abstract / Description of output

Conference

Access to Document

Fingerprint

Projects

ISP 2017/22-Dunn Ian

Cute-Egg, improvement of eggshell cuticle quality to reduce vertical transmission of zoonotic and pathogenic organisms

Control of development and reproductive traits

Cite this