Bovine Granulosa Cell Culture

Bushra T Mohammed, F Xavier Donadeu

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review


Culture of granulosa cells has for long provided a useful tool to understand the molecular processes underlying ovarian follicle development. Among all species investigated, cattle have become an excellent model for in vitro studies on follicular biology, both because of their resemblance with humans in terms of follicular biology and the importance of reproductive failure as a cause of lost productivity in the dairy industry. In this chapter, we describe up-to-date methods for the harvesting of granulosa cells from bovine ovaries collected post-mortem, as well as procedures for both culturing granulosa cells in an undifferentiated state and inducing their luteinization in vitro, and for the efficient transfection of granulosa cells with oligonucleotide sequences for the purpose of investigating the function of specific genes in vitro.

Original languageEnglish
Title of host publicationEpithelial Cell Culture
Subtitle of host publicationMethods in Molecular Biology
PublisherSpringer Press
Number of pages9
Publication statusE-pub ahead of print - 30 Jun 2018

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745


  • Cow
  • Granulosa cells
  • In vitro luteinization
  • Transfection


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