Bovine intelectins: cDNA sequencing and expression in the bovine intestine

Stacey C Blease, Anne T French, Pamela A Knight, David L Gally, Alan D Pemberton

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Intelectins (Itlns) are lectins with potential roles in innate immunity, capable of binding bacteria via galactofuranose residues. Itlns also function as intestinal receptors for the antimicrobial glycoprotein lactoferrin (Lf). Since Lf binds strongly to enterohemorrhagic Escherichia coli O157:H7 (EHEC), we aimed to determine the expression of Lf receptor in terminal rectum, the site of predilection of EHEC in cattle. We sequenced two bovine intelectins (Itln1 and Itln2) and showed that both were expressed in abomasum and rectum, but expression appeared minimal in the jejunum. There was significantly higher expression of Itln2 in terminal rather than proximal rectum. Lactoferrin was expressed in all samples examined. Thus, we have demonstrated two novel bovine Itlns and shown that they are expressed along with Lf in the gastrointestinal tract, where they may interact with microbial pathogens.
Original languageEnglish
Pages (from-to)254-6
Number of pages3
JournalResearch in Veterinary Science
Volume86
Issue number2
DOIs
Publication statusPublished - Apr 2009

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