Brain-derived neurotrophic factor is a regulator of human oocyte maturation and early embryo development

Richard A. Anderson; Rosemary A. L. Bayne; John Gardner; Paul A. De Sousa

doi:10.1016/j.fertnstert.2009.04.007

Brain-derived neurotrophic factor is a regulator of human oocyte maturation and early embryo development

Richard A. Anderson, Rosemary A. L. Bayne, John Gardner, Paul A. De Sousa

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To investigate a role for brain-derived neurotrophic factor (BDNF) in human oocyte maturation.

Design: Prospective study.

Setting: Research institute.

Patients: Women undergoing laparoscopic sterilization.

Intervention(s): Small antral follicle cumulus oocyte complexes (COCs) were matured in vitro (IVM) to metaphase II (MII) in media with hormones (H; FSH, LH, E-2), serum replacement (SR). BDNF, or blocking antibodies to BDNF (BDNF/AB and TrkB/Fc), and activated.

Main Outcome Measure(s): The COCs were analyzed for expression of neurotrophin ligands/receptors and cumulus genes (HAS2, TNFAIP6, PTGS2, GREM1) by reverse transcription polymerase chain reaction (RT-PCR), cumulus expansion, maturation to MII, and parthenogenetic embryo development.

Result(s): The BDNF and truncated TrkB receptor were expressed in cumulus and mature oocytes. There was no difference in MII yields after IVM in control (H + SR) versus H + BDNF, H + SR + BDNF, or BDNF + SR media. However, both BDNF/AB and TrkB/Fc improved MII yields. After activation, normal cleavage was highest in H + SR (38%), whereas blocking antibodies yielded the highest abnormal cleavage (BDNF/AB 68%; TrkB/Fc 57%). Failure to cleave was highest in H + BDNF + SR (92%). Only H + SR yielded morulae/blastocysts (6%). Expression of GREM1 in cumulus increased after IVM in H + BDNF versus H + SR or in vivo maturation.

Original language	English
Pages (from-to)	1394-1406
Number of pages	13
Journal	Fertility and Sterility
Volume	93
Issue number	5
DOIs	https://doi.org/10.1016/j.fertnstert.2009.04.007
Publication status	Published - 15 Mar 2010

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10.1016/j.fertnstert.2009.04.007

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@article{5b12269ed273474ea451de3b384506c8,

title = "Brain-derived neurotrophic factor is a regulator of human oocyte maturation and early embryo development",

abstract = "Objective: To investigate a role for brain-derived neurotrophic factor (BDNF) in human oocyte maturation.Design: Prospective study.Setting: Research institute.Patients: Women undergoing laparoscopic sterilization.Intervention(s): Small antral follicle cumulus oocyte complexes (COCs) were matured in vitro (IVM) to metaphase II (MII) in media with hormones (H; FSH, LH, E-2), serum replacement (SR). BDNF, or blocking antibodies to BDNF (BDNF/AB and TrkB/Fc), and activated.Main Outcome Measure(s): The COCs were analyzed for expression of neurotrophin ligands/receptors and cumulus genes (HAS2, TNFAIP6, PTGS2, GREM1) by reverse transcription polymerase chain reaction (RT-PCR), cumulus expansion, maturation to MII, and parthenogenetic embryo development.Result(s): The BDNF and truncated TrkB receptor were expressed in cumulus and mature oocytes. There was no difference in MII yields after IVM in control (H + SR) versus H + BDNF, H + SR + BDNF, or BDNF + SR media. However, both BDNF/AB and TrkB/Fc improved MII yields. After activation, normal cleavage was highest in H + SR (38\%), whereas blocking antibodies yielded the highest abnormal cleavage (BDNF/AB 68\%; TrkB/Fc 57\%). Failure to cleave was highest in H + BDNF + SR (92\%). Only H + SR yielded morulae/blastocysts (6\%). Expression of GREM1 in cumulus increased after IVM in H + BDNF versus H + SR or in vivo maturation.Conclusion(s): The BDNF signaling within COCs influences oocyte maturation and early embryogenesis. (Fertil Steril (R) 2010;93:1394-406. (C)2010 by American Society for Reproductive Medicine.)",

author = "Anderson, \{Richard A.\} and Bayne, \{Rosemary A. L.\} and John Gardner and \{De Sousa\}, \{Paul A.\}",

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doi = "10.1016/j.fertnstert.2009.04.007",

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TY - JOUR

T1 - Brain-derived neurotrophic factor is a regulator of human oocyte maturation and early embryo development

AU - Anderson, Richard A.

AU - Bayne, Rosemary A. L.

AU - Gardner, John

AU - De Sousa, Paul A.

PY - 2010/3/15

Y1 - 2010/3/15

N2 - Objective: To investigate a role for brain-derived neurotrophic factor (BDNF) in human oocyte maturation.Design: Prospective study.Setting: Research institute.Patients: Women undergoing laparoscopic sterilization.Intervention(s): Small antral follicle cumulus oocyte complexes (COCs) were matured in vitro (IVM) to metaphase II (MII) in media with hormones (H; FSH, LH, E-2), serum replacement (SR). BDNF, or blocking antibodies to BDNF (BDNF/AB and TrkB/Fc), and activated.Main Outcome Measure(s): The COCs were analyzed for expression of neurotrophin ligands/receptors and cumulus genes (HAS2, TNFAIP6, PTGS2, GREM1) by reverse transcription polymerase chain reaction (RT-PCR), cumulus expansion, maturation to MII, and parthenogenetic embryo development.Result(s): The BDNF and truncated TrkB receptor were expressed in cumulus and mature oocytes. There was no difference in MII yields after IVM in control (H + SR) versus H + BDNF, H + SR + BDNF, or BDNF + SR media. However, both BDNF/AB and TrkB/Fc improved MII yields. After activation, normal cleavage was highest in H + SR (38%), whereas blocking antibodies yielded the highest abnormal cleavage (BDNF/AB 68%; TrkB/Fc 57%). Failure to cleave was highest in H + BDNF + SR (92%). Only H + SR yielded morulae/blastocysts (6%). Expression of GREM1 in cumulus increased after IVM in H + BDNF versus H + SR or in vivo maturation.Conclusion(s): The BDNF signaling within COCs influences oocyte maturation and early embryogenesis. (Fertil Steril (R) 2010;93:1394-406. (C)2010 by American Society for Reproductive Medicine.)

AB - Objective: To investigate a role for brain-derived neurotrophic factor (BDNF) in human oocyte maturation.Design: Prospective study.Setting: Research institute.Patients: Women undergoing laparoscopic sterilization.Intervention(s): Small antral follicle cumulus oocyte complexes (COCs) were matured in vitro (IVM) to metaphase II (MII) in media with hormones (H; FSH, LH, E-2), serum replacement (SR). BDNF, or blocking antibodies to BDNF (BDNF/AB and TrkB/Fc), and activated.Main Outcome Measure(s): The COCs were analyzed for expression of neurotrophin ligands/receptors and cumulus genes (HAS2, TNFAIP6, PTGS2, GREM1) by reverse transcription polymerase chain reaction (RT-PCR), cumulus expansion, maturation to MII, and parthenogenetic embryo development.Result(s): The BDNF and truncated TrkB receptor were expressed in cumulus and mature oocytes. There was no difference in MII yields after IVM in control (H + SR) versus H + BDNF, H + SR + BDNF, or BDNF + SR media. However, both BDNF/AB and TrkB/Fc improved MII yields. After activation, normal cleavage was highest in H + SR (38%), whereas blocking antibodies yielded the highest abnormal cleavage (BDNF/AB 68%; TrkB/Fc 57%). Failure to cleave was highest in H + BDNF + SR (92%). Only H + SR yielded morulae/blastocysts (6%). Expression of GREM1 in cumulus increased after IVM in H + BDNF versus H + SR or in vivo maturation.Conclusion(s): The BDNF signaling within COCs influences oocyte maturation and early embryogenesis. (Fertil Steril (R) 2010;93:1394-406. (C)2010 by American Society for Reproductive Medicine.)

UR - https://www.scopus.com/pages/publications/77949262397

U2 - 10.1016/j.fertnstert.2009.04.007

DO - 10.1016/j.fertnstert.2009.04.007

M3 - Article

SN - 0015-0282

VL - 93

SP - 1394

EP - 1406

JO - Fertility and Sterility

JF - Fertility and Sterility

IS - 5

ER -

Brain-derived neurotrophic factor is a regulator of human oocyte maturation and early embryo development

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