Bridging and Clumping: Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria

Samuel Crocodile Wassmer; Georges Emile Raymond Grau; Ian James Callum MacCormick

doi:10.1007/978-1-0716-2189-9_38

Bridging and Clumping: Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria

Samuel Crocodile Wassmer, Georges Emile Raymond Grau, Ian James Callum MacCormick^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract / Description of output

The methods presented in this chapter describe how to perform ex vivo clumping and in vitro bridging assays in the context of cerebral malaria. Both the protocols are detailed, and emphasis is made on how to prepare platelet suspensions suitable to each technique, including description of specific buffers and reagents to minimize the risk of aggregation while maintaining the platelet properties.

Original language	English
Title of host publication	Malaria Immunology
Subtitle of host publication	Targeting the Surface of Infected Erythrocytes
Editors	Anja Tatiana Ramstedt Jensen, Lars Hviid
Place of Publication	New York
Publisher	Humana Press
Pages	505-514
Number of pages	10
Volume	2470
ISBN (Electronic)	978-1-0716-2189-9
ISBN (Print)	978-1-0716-2188-2
DOIs	https://doi.org/10.1007/978-1-0716-2189-9_38
Publication status	Published - 27 Jul 2022

Publication series

Name	Methods in molecular biology (Clifton, N.J.)
Publisher	Humana Press
ISSN (Print)	1064-3745

Keywords / Materials (for Non-textual outputs)

Cell Adhesion
Endothelial Cells
Erythrocytes
Humans
Malaria, Cerebral
Malaria, Falciparum
Plasmodium falciparum

Access to Document

10.1007/978-1-0716-2189-9_38

Cite this

Wassmer, S. C., Grau, G. E. R., & MacCormick, I. J. C. (2022). Bridging and Clumping: Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria. In A. T. Ramstedt Jensen, & L. Hviid (Eds.), Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 505-514). (Methods in molecular biology (Clifton, N.J.)). Humana Press. https://doi.org/10.1007/978-1-0716-2189-9_38

Wassmer, Samuel Crocodile ; Grau, Georges Emile Raymond ; MacCormick, Ian James Callum. / Bridging and Clumping : Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. editor / Anja Tatiana Ramstedt Jensen ; Lars Hviid. Vol. 2470 New York : Humana Press, 2022. pp. 505-514 (Methods in molecular biology (Clifton, N.J.)).

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title = "Bridging and Clumping: Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria",

abstract = "The methods presented in this chapter describe how to perform ex vivo clumping and in vitro bridging assays in the context of cerebral malaria. Both the protocols are detailed, and emphasis is made on how to prepare platelet suspensions suitable to each technique, including description of specific buffers and reagents to minimize the risk of aggregation while maintaining the platelet properties.",

keywords = "Cell Adhesion, Endothelial Cells, Erythrocytes, Humans, Malaria, Cerebral, Malaria, Falciparum, Plasmodium falciparum",

author = "Wassmer, {Samuel Crocodile} and Grau, {Georges Emile Raymond} and MacCormick, {Ian James Callum}",

note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

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language = "English",

isbn = "978-1-0716-2188-2",

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editor = "{Ramstedt Jensen}, {Anja Tatiana} and Lars Hviid",

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Wassmer, SC, Grau, GER & MacCormick, IJC 2022, Bridging and Clumping: Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria. in AT Ramstedt Jensen & L Hviid (eds), Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Methods in molecular biology (Clifton, N.J.), Humana Press, New York, pp. 505-514. https://doi.org/10.1007/978-1-0716-2189-9_38

Bridging and Clumping: Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria. / Wassmer, Samuel Crocodile; Grau, Georges Emile Raymond; MacCormick, Ian James Callum.
Malaria Immunology: Targeting the Surface of Infected Erythrocytes. ed. / Anja Tatiana Ramstedt Jensen; Lars Hviid. Vol. 2470 New York: Humana Press, 2022. p. 505-514 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Bridging and Clumping

T2 - Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria

AU - Wassmer, Samuel Crocodile

AU - Grau, Georges Emile Raymond

AU - MacCormick, Ian James Callum

PY - 2022/7/27

Y1 - 2022/7/27

N2 - The methods presented in this chapter describe how to perform ex vivo clumping and in vitro bridging assays in the context of cerebral malaria. Both the protocols are detailed, and emphasis is made on how to prepare platelet suspensions suitable to each technique, including description of specific buffers and reagents to minimize the risk of aggregation while maintaining the platelet properties.

AB - The methods presented in this chapter describe how to perform ex vivo clumping and in vitro bridging assays in the context of cerebral malaria. Both the protocols are detailed, and emphasis is made on how to prepare platelet suspensions suitable to each technique, including description of specific buffers and reagents to minimize the risk of aggregation while maintaining the platelet properties.

KW - Cell Adhesion

KW - Endothelial Cells

KW - Erythrocytes

KW - Humans

KW - Malaria, Cerebral

KW - Malaria, Falciparum

KW - Plasmodium falciparum

U2 - 10.1007/978-1-0716-2189-9_38

DO - 10.1007/978-1-0716-2189-9_38

M3 - Chapter (peer-reviewed)

C2 - 35881370

SN - 978-1-0716-2188-2

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 505

EP - 514

BT - Malaria Immunology

A2 - Ramstedt Jensen, Anja Tatiana

A2 - Hviid, Lars

PB - Humana Press

CY - New York

ER -

Wassmer SC, Grau GER, MacCormick IJC. Bridging and Clumping: Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria. In Ramstedt Jensen AT, Hviid L, editors, Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. New York: Humana Press. 2022. p. 505-514. (Methods in molecular biology (Clifton, N.J.)). doi: 10.1007/978-1-0716-2189-9_38

Bridging and Clumping: Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria

Abstract / Description of output

Publication series

Keywords / Materials (for Non-textual outputs)

Access to Document

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Cite this