Brucella Species Circulating in Smallholder Dairy Cattle in Tanzania

Isaac Joseph  Mengele; James Miser  Akoko; Gabriel Mkilema  Shirima; Shedrack Festo  Bwatota; Shabani Kiyabo  Motto; Luis E. Hernandez-Castro; Daniel Mushumbusi  Komwihangilo; Eliamoni  Lyatuu; Mark Bronsvoort; Elizabeth Anne Jessie Cook

doi:10.3390/pathogens13090815

Brucella Species Circulating in Smallholder Dairy Cattle in Tanzania

Isaac Joseph Mengele , James Miser Akoko, Gabriel Mkilema Shirima, Shedrack Festo Bwatota, Shabani Kiyabo Motto, Luis E. Hernandez-Castro, Daniel Mushumbusi Komwihangilo , Eliamoni Lyatuu , Mark Bronsvoort, Elizabeth Anne Jessie Cook

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Brucellosis is a zoonosis caused by bacteria of the genus Brucella, which results in economic
losses relating to livestock and threatens public health. A cross-sectional study was conducted to determine the molecular prevalence of Brucella species in smallholder dairy cattle in six regions of Tanzania from July 2019 to October 2020. Dairy cattle (n = 2048) were sampled from 1371 farms. DNA extracted from blood and vaginal swabs was tested for Brucella using qPCR targeting the IS711 gene and positives were tested for the alkB marker for B. abortus and BMEI1172 marker for B. melitensis. The molecular prevalence was 3.5% (95% CI: 2.8–4.4) with the highest prevalence 8.1% (95% CI:4.6–13.0) in Njombe region. B. melitensis was the predominant species detected (66.2%). Further studies are recommended to understand the source of B. melitensis and its implications for veterinary public health. Livestock keepers should be informed of the risks and biosecurity practices to reduce the introduction and control of Brucella. Cattle and small ruminant vaccination programs could be implemented to control brucellosis in high-risk populations in the country

Original language	English
Article number	815
Pages (from-to)	1-12
Number of pages	12
Journal	Pathogens
Volume	13
Issue number	9
Early online date	21 Sept 2024
DOIs	https://doi.org/10.3390/pathogens13090815
Publication status	Published - 21 Sept 2024

Keywords / Materials (for Non-textual outputs)

dairy cattle
brucellosis
qPCR
molecular prevalence
Brucella
Tanzania

Access to Document

10.3390/pathogens13090815

pathogens-13-00815Final published version, 1.11 MBLicence: Creative Commons: Attribution (CC-BY)

High resolution genotypes and phenotypes associated with livestock disease resistance and survival
Bronsvoort, M.
Bill and Melinda Gates Foundation
1/10/15 → 31/03/23
Project: Research
CTLGH: A global shared data biological sample resource to support productivity improvement for tropical livestock
Freeman, P., Bronsvoort, M., Clark, E., Connelley, T., Hassan, M., McGrew, M., Morrison, L., Prendergast, J., Robert, C., Smith, J., Sparks, N., Watson, M. & Whitelaw, B.
Non-EU-based charities
1/10/15 → 31/03/22
Project: Research

Cite this

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title = "Brucella Species Circulating in Smallholder Dairy Cattle in Tanzania",

abstract = "Brucellosis is a zoonosis caused by bacteria of the genus Brucella, which results in economiclosses relating to livestock and threatens public health. A cross-sectional study was conducted to determine the molecular prevalence of Brucella species in smallholder dairy cattle in six regions of Tanzania from July 2019 to October 2020. Dairy cattle (n = 2048) were sampled from 1371 farms. DNA extracted from blood and vaginal swabs was tested for Brucella using qPCR targeting the IS711 gene and positives were tested for the alkB marker for B. abortus and BMEI1172 marker for B. melitensis. The molecular prevalence was 3.5% (95% CI: 2.8–4.4) with the highest prevalence 8.1% (95% CI:4.6–13.0) in Njombe region. B. melitensis was the predominant species detected (66.2%). Further studies are recommended to understand the source of B. melitensis and its implications for veterinary public health. Livestock keepers should be informed of the risks and biosecurity practices to reduce the introduction and control of Brucella. Cattle and small ruminant vaccination programs could be implemented to control brucellosis in high-risk populations in the country",

keywords = "dairy cattle, brucellosis, qPCR, molecular prevalence, Brucella, Tanzania",

author = "Mengele, {Isaac Joseph} and Akoko, {James Miser} and Shirima, {Gabriel Mkilema} and Bwatota, {Shedrack Festo} and Motto, {Shabani Kiyabo} and Hernandez-Castro, {Luis E.} and Komwihangilo, {Daniel Mushumbusi} and Eliamoni Lyatuu and Mark Bronsvoort and Cook, {Elizabeth Anne Jessie}",

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AU - Mengele , Isaac Joseph

AU - Akoko, James Miser

AU - Shirima, Gabriel Mkilema

AU - Bwatota, Shedrack Festo

AU - Motto, Shabani Kiyabo

AU - Hernandez-Castro, Luis E.

AU - Komwihangilo , Daniel Mushumbusi

AU - Lyatuu , Eliamoni

AU - Bronsvoort, Mark

AU - Cook, Elizabeth Anne Jessie

PY - 2024/9/21

Y1 - 2024/9/21

N2 - Brucellosis is a zoonosis caused by bacteria of the genus Brucella, which results in economiclosses relating to livestock and threatens public health. A cross-sectional study was conducted to determine the molecular prevalence of Brucella species in smallholder dairy cattle in six regions of Tanzania from July 2019 to October 2020. Dairy cattle (n = 2048) were sampled from 1371 farms. DNA extracted from blood and vaginal swabs was tested for Brucella using qPCR targeting the IS711 gene and positives were tested for the alkB marker for B. abortus and BMEI1172 marker for B. melitensis. The molecular prevalence was 3.5% (95% CI: 2.8–4.4) with the highest prevalence 8.1% (95% CI:4.6–13.0) in Njombe region. B. melitensis was the predominant species detected (66.2%). Further studies are recommended to understand the source of B. melitensis and its implications for veterinary public health. Livestock keepers should be informed of the risks and biosecurity practices to reduce the introduction and control of Brucella. Cattle and small ruminant vaccination programs could be implemented to control brucellosis in high-risk populations in the country

AB - Brucellosis is a zoonosis caused by bacteria of the genus Brucella, which results in economiclosses relating to livestock and threatens public health. A cross-sectional study was conducted to determine the molecular prevalence of Brucella species in smallholder dairy cattle in six regions of Tanzania from July 2019 to October 2020. Dairy cattle (n = 2048) were sampled from 1371 farms. DNA extracted from blood and vaginal swabs was tested for Brucella using qPCR targeting the IS711 gene and positives were tested for the alkB marker for B. abortus and BMEI1172 marker for B. melitensis. The molecular prevalence was 3.5% (95% CI: 2.8–4.4) with the highest prevalence 8.1% (95% CI:4.6–13.0) in Njombe region. B. melitensis was the predominant species detected (66.2%). Further studies are recommended to understand the source of B. melitensis and its implications for veterinary public health. Livestock keepers should be informed of the risks and biosecurity practices to reduce the introduction and control of Brucella. Cattle and small ruminant vaccination programs could be implemented to control brucellosis in high-risk populations in the country

KW - dairy cattle

KW - brucellosis

KW - qPCR

KW - molecular prevalence

KW - Brucella

KW - Tanzania

U2 - 10.3390/pathogens13090815

DO - 10.3390/pathogens13090815

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ER -

Brucella Species Circulating in Smallholder Dairy Cattle in Tanzania

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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Projects

High resolution genotypes and phenotypes associated with livestock disease resistance and survival

CTLGH: A global shared data biological sample resource to support productivity improvement for tropical livestock

Cite this