Building in vitro tools for livestock genomics: chromosomal variation within the PK15 cell line

M Johnsson, J M Hickey, M K Jungnickel

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

BACKGROUND: Cultured porcine cell lines are powerful tools for functional genomics and in vitro phenotypic testing of candidate causal variants. However, to be utilised for genomic or variant interrogation assays, the genome sequence and structure of cultured cell lines must be realised. In this work, we called variants and used read coverage in combination with within-sample allele frequency to detect potential aneuploidy in two immortalised porcine kidney epithelial (PK15) cell lines and in a pig embryonic fibroblast line.

RESULTS: We compared two PK15 cultured cells samples: a new American Type Culture Collection (ATCC) sample and one that has been utilised and passaged within the laboratory for an extended period (> 10 years). Read coverage and within-sample allele frequencies showed that several chromosomes are fully or partially aneuploid in both PK15 lines, including potential trisomy of chromosome 4 and tetrasomy of chromosome 17. The older PK15 line showed evidence of additional structural variation and potentially clonal variation. By comparison, the pig embryonic fibroblast line was free from the gross aneuploidies seen in the PK15s.

CONCLUSIONS: Our results show that the PK15 cell lines examined have aneuploidies and complex structural variants in their genomes. We propose that screening for aneuploidy should be considered for cell lines, and discuss implications for livestock genomics.

Original languageEnglish
Article number49
Pages (from-to)1-11
Number of pages11
JournalBMC Genomics
Issue number1
Early online date11 Jan 2024
Publication statusE-pub ahead of print - 11 Jan 2024

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Swine/genetics
  • Livestock
  • Genomics
  • Cell Line
  • Aneuploidy
  • Chromosomes


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