Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules

S. Suparak, V. Muangsombut, D. Riyapa, J.M. Stevens, M.P. Stevens, G. Lertmemongkolchai, S. Korbsrisate

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Burkholderia pseudomallei induces the formation of multinucleated giant cells in cell monolayers. After infection of human macrophage-like U937 cells with B. pseudomallei, addition of monoclonal antibodies against integrin-associated protein (CD47), E-selectin (CD62E), a fusion regulatory protein (CD98), and E-cadherin (CD324) suppressed multinucleated giant cells in a concentration-dependent manner while monoclonal antibodies against other surface molecules did not inhibit fusion despite binding to the cell surface. Flow cytometric analysis showed increased expression of CD47 and CD98, but not CD62E and CD324, upon B. pseudomallei infection. Our data suggest the involvement of specific cellular factors in the process of B. pseudomallei-induced fusion.
Original languageUndefined/Unknown
Pages (from-to)1006-1011
Number of pages6
JournalMicrobes and Infection
Volume13
Issue number12-13
Publication statusPublished - 2011

Keywords / Materials (for Non-textual outputs)

  • Burkholderia pseudomallei U937 cell Multinucleated giant cell CD47 CD98

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