Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules

S. Suparak; V. Muangsombut; D. Riyapa; J.M. Stevens; M.P. Stevens; G. Lertmemongkolchai; S. Korbsrisate

Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules

S. Suparak, V. Muangsombut, D. Riyapa, J.M. Stevens, M.P. Stevens, G. Lertmemongkolchai, S. Korbsrisate

Research output: Contribution to journal › Article › peer-review

Abstract

Burkholderia pseudomallei induces the formation of multinucleated giant cells in cell monolayers. After infection of human macrophage-like U937 cells with B. pseudomallei, addition of monoclonal antibodies against integrin-associated protein (CD47), E-selectin (CD62E), a fusion regulatory protein (CD98), and E-cadherin (CD324) suppressed multinucleated giant cells in a concentration-dependent manner while monoclonal antibodies against other surface molecules did not inhibit fusion despite binding to the cell surface. Flow cytometric analysis showed increased expression of CD47 and CD98, but not CD62E and CD324, upon B. pseudomallei infection. Our data suggest the involvement of specific cellular factors in the process of B. pseudomallei-induced fusion.

Original language	Undefined/Unknown
Pages (from-to)	1006-1011
Number of pages	6
Journal	Microbes and Infection
Volume	13
Issue number	12-13
Publication status	Published - 2011

Keywords / Materials (for Non-textual outputs)

Burkholderia pseudomallei U937 cell Multinucleated giant cell CD47 CD98

Access to Document

http://www.sciencedirect.com/science/article/pii/S1286457911001663

Cite this

Suparak, S., Muangsombut, V., Riyapa, D., Stevens, J. M., Stevens, M. P., Lertmemongkolchai, G., & Korbsrisate, S. (2011). Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules. Microbes and Infection, 13(12-13), 1006-1011. http://www.sciencedirect.com/science/article/pii/S1286457911001663

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title = "Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules",

abstract = "Burkholderia pseudomallei induces the formation of multinucleated giant cells in cell monolayers. After infection of human macrophage-like U937 cells with B. pseudomallei, addition of monoclonal antibodies against integrin-associated protein (CD47), E-selectin (CD62E), a fusion regulatory protein (CD98), and E-cadherin (CD324) suppressed multinucleated giant cells in a concentration-dependent manner while monoclonal antibodies against other surface molecules did not inhibit fusion despite binding to the cell surface. Flow cytometric analysis showed increased expression of CD47 and CD98, but not CD62E and CD324, upon B. pseudomallei infection. Our data suggest the involvement of specific cellular factors in the process of B. pseudomallei-induced fusion.",

keywords = "Burkholderia pseudomallei U937 cell Multinucleated giant cell CD47 CD98",

author = "S. Suparak and V. Muangsombut and D. Riyapa and J.M. Stevens and M.P. Stevens and G. Lertmemongkolchai and S. Korbsrisate",

year = "2011",

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Suparak, S, Muangsombut, V, Riyapa, D, Stevens, JM , Stevens, MP, Lertmemongkolchai, G & Korbsrisate, S 2011, 'Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules', Microbes and Infection, vol. 13, no. 12-13, pp. 1006-1011. <http://www.sciencedirect.com/science/article/pii/S1286457911001663>

TY - JOUR

T1 - Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules

AU - Suparak, S.

AU - Muangsombut, V.

AU - Riyapa, D.

AU - Stevens, J.M.

AU - Stevens, M.P.

AU - Lertmemongkolchai, G.

AU - Korbsrisate, S.

PY - 2011

Y1 - 2011

N2 - Burkholderia pseudomallei induces the formation of multinucleated giant cells in cell monolayers. After infection of human macrophage-like U937 cells with B. pseudomallei, addition of monoclonal antibodies against integrin-associated protein (CD47), E-selectin (CD62E), a fusion regulatory protein (CD98), and E-cadherin (CD324) suppressed multinucleated giant cells in a concentration-dependent manner while monoclonal antibodies against other surface molecules did not inhibit fusion despite binding to the cell surface. Flow cytometric analysis showed increased expression of CD47 and CD98, but not CD62E and CD324, upon B. pseudomallei infection. Our data suggest the involvement of specific cellular factors in the process of B. pseudomallei-induced fusion.

AB - Burkholderia pseudomallei induces the formation of multinucleated giant cells in cell monolayers. After infection of human macrophage-like U937 cells with B. pseudomallei, addition of monoclonal antibodies against integrin-associated protein (CD47), E-selectin (CD62E), a fusion regulatory protein (CD98), and E-cadherin (CD324) suppressed multinucleated giant cells in a concentration-dependent manner while monoclonal antibodies against other surface molecules did not inhibit fusion despite binding to the cell surface. Flow cytometric analysis showed increased expression of CD47 and CD98, but not CD62E and CD324, upon B. pseudomallei infection. Our data suggest the involvement of specific cellular factors in the process of B. pseudomallei-induced fusion.

KW - Burkholderia pseudomallei U937 cell Multinucleated giant cell CD47 CD98

M3 - Article

SN - 1769-714X

VL - 13

SP - 1006

EP - 1011

JO - Microbes and Infection

JF - Microbes and Infection

IS - 12-13

ER -