C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes

N. J. Peake, D. L. Bader, S. Vessillier, M. Ramachandran, D. M. Salter, A. J. Hobbs, T. T. Chowdhury*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Signals induced by mechanical loading and C-type natriuretic peptide (CNP) represent chondroprotective routes that may potentially prevent osteoarthritis (OA). We examined whether CNP will reduce hyaluronan production and export via members of the multidrug resistance protein (MRP) and diminish pro-inflammatory effects in human chondrocytes. The presence of interleukin-1β (IL-1β) increased HA production and export via MRP5 that was reduced with CNP and/or loading. Treatment with IL-1β conditioned medium increased production of catabolic mediators and the response was reduced with the hyaluronan inhibitor, Pep-1. The induction of pro-inflammatory cytokines by the conditioned medium was reduced by CNP and/or Pep-1, αCD44 or αTLR4 in a cytokine-dependent manner, suggesting that the CNP pathway is protective and should be exploited further.

Original languageEnglish
Pages (from-to)784-789
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume465
Issue number4
DOIs
Publication statusPublished - 19 Aug 2015

Keywords

  • C-type natriuretic peptide
  • Chondrocyte
  • Interleukin-1β
  • Mechanical loading
  • Osteoarthritis

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