C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes

N. J. Peake; D. L. Bader; S. Vessillier; M. Ramachandran; D. M. Salter; A. J. Hobbs; T. T. Chowdhury

doi:10.1016/j.bbrc.2015.08.087

C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes

N. J. Peake, D. L. Bader, S. Vessillier, M. Ramachandran, D. M. Salter, A. J. Hobbs, T. T. Chowdhury^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Signals induced by mechanical loading and C-type natriuretic peptide (CNP) represent chondroprotective routes that may potentially prevent osteoarthritis (OA). We examined whether CNP will reduce hyaluronan production and export via members of the multidrug resistance protein (MRP) and diminish pro-inflammatory effects in human chondrocytes. The presence of interleukin-1β (IL-1β) increased HA production and export via MRP5 that was reduced with CNP and/or loading. Treatment with IL-1β conditioned medium increased production of catabolic mediators and the response was reduced with the hyaluronan inhibitor, Pep-1. The induction of pro-inflammatory cytokines by the conditioned medium was reduced by CNP and/or Pep-1, αCD44 or αTLR4 in a cytokine-dependent manner, suggesting that the CNP pathway is protective and should be exploited further.

Original language	English
Pages (from-to)	784-789
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	465
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2015.08.087
Publication status	Published - 19 Aug 2015

Keywords / Materials (for Non-textual outputs)

C-type natriuretic peptide
Chondrocyte
Interleukin-1β
Mechanical loading
Osteoarthritis

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10.1016/j.bbrc.2015.08.087

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title = "C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes",

abstract = "Signals induced by mechanical loading and C-type natriuretic peptide (CNP) represent chondroprotective routes that may potentially prevent osteoarthritis (OA). We examined whether CNP will reduce hyaluronan production and export via members of the multidrug resistance protein (MRP) and diminish pro-inflammatory effects in human chondrocytes. The presence of interleukin-1β (IL-1β) increased HA production and export via MRP5 that was reduced with CNP and/or loading. Treatment with IL-1β conditioned medium increased production of catabolic mediators and the response was reduced with the hyaluronan inhibitor, Pep-1. The induction of pro-inflammatory cytokines by the conditioned medium was reduced by CNP and/or Pep-1, αCD44 or αTLR4 in a cytokine-dependent manner, suggesting that the CNP pathway is protective and should be exploited further.",

keywords = "C-type natriuretic peptide, Chondrocyte, Interleukin-1β, Mechanical loading, Osteoarthritis",

author = "Peake, {N. J.} and Bader, {D. L.} and S. Vessillier and M. Ramachandran and Salter, {D. M.} and Hobbs, {A. J.} and Chowdhury, {T. T.}",

note = "Date of Acceptance: 20/08/2015",

year = "2015",

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day = "19",

doi = "10.1016/j.bbrc.2015.08.087",

language = "English",

volume = "465",

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T1 - C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes

AU - Peake, N. J.

AU - Bader, D. L.

AU - Vessillier, S.

AU - Ramachandran, M.

AU - Salter, D. M.

AU - Hobbs, A. J.

AU - Chowdhury, T. T.

N1 - Date of Acceptance: 20/08/2015

PY - 2015/8/19

Y1 - 2015/8/19

N2 - Signals induced by mechanical loading and C-type natriuretic peptide (CNP) represent chondroprotective routes that may potentially prevent osteoarthritis (OA). We examined whether CNP will reduce hyaluronan production and export via members of the multidrug resistance protein (MRP) and diminish pro-inflammatory effects in human chondrocytes. The presence of interleukin-1β (IL-1β) increased HA production and export via MRP5 that was reduced with CNP and/or loading. Treatment with IL-1β conditioned medium increased production of catabolic mediators and the response was reduced with the hyaluronan inhibitor, Pep-1. The induction of pro-inflammatory cytokines by the conditioned medium was reduced by CNP and/or Pep-1, αCD44 or αTLR4 in a cytokine-dependent manner, suggesting that the CNP pathway is protective and should be exploited further.

AB - Signals induced by mechanical loading and C-type natriuretic peptide (CNP) represent chondroprotective routes that may potentially prevent osteoarthritis (OA). We examined whether CNP will reduce hyaluronan production and export via members of the multidrug resistance protein (MRP) and diminish pro-inflammatory effects in human chondrocytes. The presence of interleukin-1β (IL-1β) increased HA production and export via MRP5 that was reduced with CNP and/or loading. Treatment with IL-1β conditioned medium increased production of catabolic mediators and the response was reduced with the hyaluronan inhibitor, Pep-1. The induction of pro-inflammatory cytokines by the conditioned medium was reduced by CNP and/or Pep-1, αCD44 or αTLR4 in a cytokine-dependent manner, suggesting that the CNP pathway is protective and should be exploited further.

KW - C-type natriuretic peptide

KW - Chondrocyte

KW - Interleukin-1β

KW - Mechanical loading

KW - Osteoarthritis

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U2 - 10.1016/j.bbrc.2015.08.087

DO - 10.1016/j.bbrc.2015.08.087

M3 - Article

AN - SCOPUS:84941315579

SN - 0006-291X

VL - 465

SP - 784

EP - 789

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes

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