Calcineurin controls the expression of numerous genes in cerebellar granule cells

Dana Kramer, Luigia Fresu, Dominique S Ashby, Tom C Freeman, Armando A Genazzani

Research output: Contribution to journalArticlepeer-review

Abstract

The Ca(2+)/calmodulin-dependent phosphatase calcineurin plays a crucial role in gene expression in different cell types such as T-lymphocytes, cardiac myocytes, and smooth muscle cells. A possible role for calcineurin in gene expression was recently found in neurons, where calcineurin regulates the expression of several genes involved in Ca(2+) homeostasis. To detect additional genes regulated in a calcineurin-dependent way in neurons we analysed gene expression profiles of cerebellar granule cells cultured in depolarising conditions in the presence or absence of the calcineurin inhibitory agents FK506 and CsA. Using oligonucleotide arrays we identified 34 genes that are differentially expressed between the samples and confirmed the calcineurin-dependent regulation of some of these genes by RT-PCR. Therefore, our results, which are likely not to be comprehensive, suggest that calcineurin plays a fundamental role in neuronal gene expression by either activating or repressing the expression of genes such as receptors, transcription factors, and signalling molecules.
Original languageEnglish
Pages (from-to)325-30
Number of pages6
JournalMolecular and Cellular Neuroscience
Volume23
Issue number2
Publication statusPublished - Jun 2003

Keywords

  • Animals
  • Animals, Newborn
  • Calcineurin
  • Calcium Signaling
  • Cell Differentiation
  • Cells, Cultured
  • Cerebellar Cortex
  • Gene Expression Regulation, Developmental
  • Membrane Potentials
  • Neurons
  • Oligonucleotide Array Sequence Analysis
  • Rats
  • Rats, Wistar
  • Receptors, Cell Surface
  • Signal Transduction
  • Tacrolimus
  • Transcription Factors

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