Can genetic bar-coding be used to identify aquatic Ranunculus L. subgenus Batrachium (DC) A. Gray? A test using some species from the British Isles

Annika Telford*, Matthew T. O'Hare, Stephen Cavers, Nigel Holmes

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Aquatic Batrachium Ranunculus species are a key component of river macrophyte communities selected for protection under European Union legislation. The group's simplified morphology and variable taxonomic interpretation often makes identification to species level very difficult. A genetic approach was trialled as an alternative, more reliable, means of identification. DNA barcoding using four markers (chloroplast and nuclear) was tested. The chloroplast sequence trnH-psbA worked best and allowed identification of three out of five species while nuclear sequences supported the identification of two hybrids. This method is amenable to simplification through techniques such as PCR-RFLP or RT-PCR. It has the potential to provide easy, rapid and inexpensive identification of Batrachium Ranunculus species. (C) 2011 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalAquatic Botany
Volume95
Issue number1
DOIs
Publication statusPublished - Jul 2011

Keywords

  • ranunculus
  • batrachium
  • DNA barcoding
  • hybrid
  • internal transcribed spacer (ITS)
  • matK
  • trnC-ycf6
  • trnH-psbA

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