Candidate cell substrates, vaccine production, and transmissible spongiform encephalopathies

Pedro Piccardo; Larisa Cervenakova; Irina Vasilyeva; Oksana Yakovleva; Igor Bacik; Juraj Cervenak; Carroll McKenzie; Lubica Kurillova; Luisa Gregori; Kitty Pomeroy; David M Asher

doi:10.3201/eid1712.110607

Candidate cell substrates, vaccine production, and transmissible spongiform encephalopathies

Pedro Piccardo, Larisa Cervenakova, Irina Vasilyeva, Oksana Yakovleva, Igor Bacik, Juraj Cervenak, Carroll McKenzie, Lubica Kurillova, Luisa Gregori, Kitty Pomeroy, David M Asher

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Transmissible spongiform encephalopathy (TSE) agents have contaminated human tissue-derived medical products, human blood components, and animal vaccines. The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. Cell lines were exposed to the infectious agents of sporadic and variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy (BSE). Exposed cultures were tested for TSE-associated prion protein (PrP(TSE)) and TSE infectivity by assay in rodents and nonhuman primates. No PrP(TSE) or infectivity has been detected in any exposed cell line under study so far. Animals inoculated with BSE brain homogenate developed typical spongiform encephalopathy. In contrast, animals inoculated with cells exposed to the BSE agent remained asymptomatic. All cell lines we studied resisted infection with 3 TSE agents, including the BSE agent.

Original language	English
Pages (from-to)	2262-9
Number of pages	8
Journal	Emerging Infectious Diseases
Volume	17
Issue number	12
DOIs	https://doi.org/10.3201/eid1712.110607
Publication status	Published - Dec 2011

Keywords / Materials (for Non-textual outputs)

Animals
Biological Assay
CHO Cells
Cattle
Cell Culture Techniques
Cell Line
Cercopithecus aethiops
Communicable Diseases, Emerging
Creutzfeldt-Jakob Syndrome
Cricetinae
Cricetulus
Disease Models, Animal
Dogs
Drug Contamination
Encephalopathy, Bovine Spongiform
HEK293 Cells
Humans
Mice
Mice, Transgenic
Prion Diseases
Prions
Saimiri
Scrapie
Vaccines
Vero Cells

Access to Document

10.3201/eid1712.110607

Cite this

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title = "Candidate cell substrates, vaccine production, and transmissible spongiform encephalopathies",

abstract = "Transmissible spongiform encephalopathy (TSE) agents have contaminated human tissue-derived medical products, human blood components, and animal vaccines. The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. Cell lines were exposed to the infectious agents of sporadic and variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy (BSE). Exposed cultures were tested for TSE-associated prion protein (PrP(TSE)) and TSE infectivity by assay in rodents and nonhuman primates. No PrP(TSE) or infectivity has been detected in any exposed cell line under study so far. Animals inoculated with BSE brain homogenate developed typical spongiform encephalopathy. In contrast, animals inoculated with cells exposed to the BSE agent remained asymptomatic. All cell lines we studied resisted infection with 3 TSE agents, including the BSE agent.",

keywords = "Animals, Biological Assay, CHO Cells, Cattle, Cell Culture Techniques, Cell Line, Cercopithecus aethiops, Communicable Diseases, Emerging, Creutzfeldt-Jakob Syndrome, Cricetinae, Cricetulus, Disease Models, Animal, Dogs, Drug Contamination, Encephalopathy, Bovine Spongiform, HEK293 Cells, Humans, Mice, Mice, Transgenic, Prion Diseases, Prions, Saimiri, Scrapie, Vaccines, Vero Cells",

author = "Pedro Piccardo and Larisa Cervenakova and Irina Vasilyeva and Oksana Yakovleva and Igor Bacik and Juraj Cervenak and Carroll McKenzie and Lubica Kurillova and Luisa Gregori and Kitty Pomeroy and Asher, {David M}",

year = "2011",

month = dec,

doi = "10.3201/eid1712.110607",

language = "English",

volume = "17",

pages = "2262--9",

journal = "Emerging Infectious Diseases",

issn = "1080-6040",

publisher = "Centers for Disease Control and Prevention",

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TY - JOUR

T1 - Candidate cell substrates, vaccine production, and transmissible spongiform encephalopathies

AU - Piccardo, Pedro

AU - Cervenakova, Larisa

AU - Vasilyeva, Irina

AU - Yakovleva, Oksana

AU - Bacik, Igor

AU - Cervenak, Juraj

AU - McKenzie, Carroll

AU - Kurillova, Lubica

AU - Gregori, Luisa

AU - Pomeroy, Kitty

AU - Asher, David M

PY - 2011/12

Y1 - 2011/12

N2 - Transmissible spongiform encephalopathy (TSE) agents have contaminated human tissue-derived medical products, human blood components, and animal vaccines. The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. Cell lines were exposed to the infectious agents of sporadic and variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy (BSE). Exposed cultures were tested for TSE-associated prion protein (PrP(TSE)) and TSE infectivity by assay in rodents and nonhuman primates. No PrP(TSE) or infectivity has been detected in any exposed cell line under study so far. Animals inoculated with BSE brain homogenate developed typical spongiform encephalopathy. In contrast, animals inoculated with cells exposed to the BSE agent remained asymptomatic. All cell lines we studied resisted infection with 3 TSE agents, including the BSE agent.

AB - Transmissible spongiform encephalopathy (TSE) agents have contaminated human tissue-derived medical products, human blood components, and animal vaccines. The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. Cell lines were exposed to the infectious agents of sporadic and variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy (BSE). Exposed cultures were tested for TSE-associated prion protein (PrP(TSE)) and TSE infectivity by assay in rodents and nonhuman primates. No PrP(TSE) or infectivity has been detected in any exposed cell line under study so far. Animals inoculated with BSE brain homogenate developed typical spongiform encephalopathy. In contrast, animals inoculated with cells exposed to the BSE agent remained asymptomatic. All cell lines we studied resisted infection with 3 TSE agents, including the BSE agent.

KW - Animals

KW - Biological Assay

KW - CHO Cells

KW - Cattle

KW - Cell Culture Techniques

KW - Cell Line

KW - Cercopithecus aethiops

KW - Communicable Diseases, Emerging

KW - Creutzfeldt-Jakob Syndrome

KW - Cricetinae

KW - Cricetulus

KW - Disease Models, Animal

KW - Dogs

KW - Drug Contamination

KW - Encephalopathy, Bovine Spongiform

KW - HEK293 Cells

KW - Humans

KW - Mice

KW - Mice, Transgenic

KW - Prion Diseases

KW - Prions

KW - Saimiri

KW - Scrapie

KW - Vaccines

KW - Vero Cells

U2 - 10.3201/eid1712.110607

DO - 10.3201/eid1712.110607

M3 - Article

C2 - 22172513

SN - 1080-6040

VL - 17

SP - 2262

EP - 2269

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 12

ER -

Candidate cell substrates, vaccine production, and transmissible spongiform encephalopathies

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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