Candidate testis-determining gene, Maestro (Mro), encodes a novel HEAT repeat protein

Lee Smith; Nick Van Hateren; John Willan; Rosario Romero; Gonzalo Blanco; Pam Siggers; James Walsh; Ruby Banerjee; Paul Denny; Chris Ponting; Andy Greenfield

doi:10.1002/dvdy.10342

Candidate testis-determining gene, Maestro (Mro), encodes a novel HEAT repeat protein

Lee Smith, Nick Van Hateren, John Willan, Rosario Romero, Gonzalo Blanco, Pam Siggers, James Walsh, Ruby Banerjee, Paul Denny, Chris Ponting, Andy Greenfield

Research output: Contribution to journal › Article › peer-review

Abstract

Mammalian sex determination depends on the presence or absence of SRY transcripts in the embryonic gonad. Expression of SRY initiates a pathway of gene expression resulting in testis development. Here, we describe a novel gene potentially functioning in this pathway using a cDNA microarray screen for genes exhibiting sexually dimorphic expression during murine gonad development. Maestro (Mro) transcripts are first detected in the developing male gonad before overt testis differentiation. By 12.5 days postcoitus (dpc), Mro transcription is restricted to the developing testis cords and its expression is not germ cell-dependent. No expression is observed in female gonads between 10.5 and 14.5 dpc. Maestro encodes a protein containing HEAT-like repeats that localizes to the nucleolus in cell transfection assays. Maestro maps to a region of mouse chromosome 18 containing a genetic modifier of XX sex reversal. We discuss the possible function of Maestro in light of these data.

Original language	English
Pages (from-to)	600-7
Number of pages	8
Journal	Developmental Dynamics
Volume	227
Issue number	4
DOIs	https://doi.org/10.1002/dvdy.10342
Publication status	Published - Aug 2003

Keywords

Amino Acid Sequence
Animals
Base Sequence
Cell Nucleolus
DNA Primers
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gonads
In Situ Hybridization, Fluorescence
Male
Mice
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Radiation Hybrid Mapping
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Sequence Analysis, DNA
Sex Differentiation

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title = "Candidate testis-determining gene, Maestro (Mro), encodes a novel HEAT repeat protein",

abstract = "Mammalian sex determination depends on the presence or absence of SRY transcripts in the embryonic gonad. Expression of SRY initiates a pathway of gene expression resulting in testis development. Here, we describe a novel gene potentially functioning in this pathway using a cDNA microarray screen for genes exhibiting sexually dimorphic expression during murine gonad development. Maestro (Mro) transcripts are first detected in the developing male gonad before overt testis differentiation. By 12.5 days postcoitus (dpc), Mro transcription is restricted to the developing testis cords and its expression is not germ cell-dependent. No expression is observed in female gonads between 10.5 and 14.5 dpc. Maestro encodes a protein containing HEAT-like repeats that localizes to the nucleolus in cell transfection assays. Maestro maps to a region of mouse chromosome 18 containing a genetic modifier of XX sex reversal. We discuss the possible function of Maestro in light of these data.",

keywords = "Amino Acid Sequence, Animals, Base Sequence, Cell Nucleolus, DNA Primers, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gonads, In Situ Hybridization, Fluorescence, Male, Mice, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Radiation Hybrid Mapping, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Sex Differentiation",

author = "Lee Smith and {Van Hateren}, Nick and John Willan and Rosario Romero and Gonzalo Blanco and Pam Siggers and James Walsh and Ruby Banerjee and Paul Denny and Chris Ponting and Andy Greenfield",

year = "2003",

month = aug,

doi = "10.1002/dvdy.10342",

language = "English",

volume = "227",

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journal = "Developmental Dynamics",

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T1 - Candidate testis-determining gene, Maestro (Mro), encodes a novel HEAT repeat protein

AU - Smith, Lee

AU - Van Hateren, Nick

AU - Willan, John

AU - Romero, Rosario

AU - Blanco, Gonzalo

AU - Siggers, Pam

AU - Walsh, James

AU - Banerjee, Ruby

AU - Denny, Paul

AU - Ponting, Chris

AU - Greenfield, Andy

PY - 2003/8

Y1 - 2003/8

N2 - Mammalian sex determination depends on the presence or absence of SRY transcripts in the embryonic gonad. Expression of SRY initiates a pathway of gene expression resulting in testis development. Here, we describe a novel gene potentially functioning in this pathway using a cDNA microarray screen for genes exhibiting sexually dimorphic expression during murine gonad development. Maestro (Mro) transcripts are first detected in the developing male gonad before overt testis differentiation. By 12.5 days postcoitus (dpc), Mro transcription is restricted to the developing testis cords and its expression is not germ cell-dependent. No expression is observed in female gonads between 10.5 and 14.5 dpc. Maestro encodes a protein containing HEAT-like repeats that localizes to the nucleolus in cell transfection assays. Maestro maps to a region of mouse chromosome 18 containing a genetic modifier of XX sex reversal. We discuss the possible function of Maestro in light of these data.

AB - Mammalian sex determination depends on the presence or absence of SRY transcripts in the embryonic gonad. Expression of SRY initiates a pathway of gene expression resulting in testis development. Here, we describe a novel gene potentially functioning in this pathway using a cDNA microarray screen for genes exhibiting sexually dimorphic expression during murine gonad development. Maestro (Mro) transcripts are first detected in the developing male gonad before overt testis differentiation. By 12.5 days postcoitus (dpc), Mro transcription is restricted to the developing testis cords and its expression is not germ cell-dependent. No expression is observed in female gonads between 10.5 and 14.5 dpc. Maestro encodes a protein containing HEAT-like repeats that localizes to the nucleolus in cell transfection assays. Maestro maps to a region of mouse chromosome 18 containing a genetic modifier of XX sex reversal. We discuss the possible function of Maestro in light of these data.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Cell Nucleolus

KW - DNA Primers

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Developmental

KW - Gonads

KW - In Situ Hybridization, Fluorescence

KW - Male

KW - Mice

KW - Molecular Sequence Data

KW - Oligonucleotide Array Sequence Analysis

KW - Radiation Hybrid Mapping

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Alignment

KW - Sequence Analysis, DNA

KW - Sex Differentiation

U2 - 10.1002/dvdy.10342

DO - 10.1002/dvdy.10342

M3 - Article

C2 - 12889070

VL - 227

SP - 600

EP - 607

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 4

ER -

Candidate testis-determining gene, Maestro (Mro), encodes a novel HEAT repeat protein

Abstract

Keywords

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