Canonical notch signaling controls the early thymic epithelial progenitor cell state and emergence of the medullary epithelial lineage in fetal thymus development

Dong Liu, Anastasia Kousa, Kathy O'Neill, Paul Rouse, Martyna Popis, Alison Farley, Simon Tomlinson, Svetlana Ulyanchenko, Francois Guillemot, Philip A Seymour, Mette Jorgensen, Palle Serup, Ute Koch, Freddy Radtke, Clare Blackburn

Research output: Contribution to journalArticlepeer-review

Abstract

Thymus function depends on the epithelial compartment of the thymic stroma. Cortical thymic epithelial cells (cTECs) regulate T cell lineage commitment and positive selection, while medullary (m) TECs impose central tolerance on the T cell repertoire. During thymus organogenesis, these functionally distinct sub-lineages are thought to arise from a common thymic epithelial progenitor cell (TEPC). The mechanisms controlling cTEC and mTEC production from the common TEPC are not however understood. Here, we show that emergence of the earliest mTEC lineage-restricted progenitors requires active NOTCH signaling in progenitor TEC and that, once specified, further mTEC development is NOTCH-independent. In addition, we demonstrate that persistent NOTCH activity favors maintenance of undifferentiated TEPC at the expense of cTEC differentiation. Finally, we uncover a cross-regulatory relationship between NOTCH and FOXN1, a master regulator of TEC differentiation. These data establish NOTCH as a potent regulator of TEPC and mTEC fate during fetal thymus development and are thus of high relevance to strategies aimed at generating/regenerating functional thymic tissue in vitro and in vivo.
Original languageEnglish
JournalDevelopment
DOIs
Publication statusPublished - 22 Jun 2020

Keywords

  • thymus
  • thymic epithelial cell
  • stem cell
  • progenitor cell
  • lineage divergence
  • differentiation
  • cell fate regulation
  • notich signaling

Fingerprint Dive into the research topics of 'Canonical notch signaling controls the early thymic epithelial progenitor cell state and emergence of the medullary epithelial lineage in fetal thymus development'. Together they form a unique fingerprint.

Cite this