Caprine PRNP polymorphisms at codons 171, 211, 222 and 240 in a Greek herd and their association with classical scrapie

Ilias G. Bouzalas, Chrysostomos I. Dovas, Georgios Banos, Maria Papanastasopoulou, Spyridon Kritas, Anna Oevermann, Dimitra Papakostaki, Chatzinasiou Evangelia, Orestis Papadopoulos, Torsten Seuberlich, Georgios Koptopoulos

Research output: Contribution to journalArticlepeer-review

Abstract

The association between PRNP variation and scrapie incidence was investigated in a highly affected Greek goat herd. Four mutations were identified at codons 171Q/R, 211R/Q, 222Q/K and 240P/S. Lysine at codon 222 was found to be associated with the protection from natural scrape (P=0 0111) Glutamine at codon 211 was observed in eight animals, all of them being scrapie-negative, indicating a possible protective role of this polymorphism although statistical analysis failed to support it (P=0.1074) A positive association (P=0 0457) between scrapie-affected goats and the wild-type Q(171)R(211)Q(222)S(240) allele is presented for the first time. In addition, a novel R(171)RQS allele, which is identical to the A(136)R(154)R(171) allele that has been associated with resistance to classical scrapie in sheep, was observed in low frequency. Resistant alleles that include K(222) and Q(211) are absent or rare in sheep and can provide the basis for the development of a feasible breeding programme for scrape eradication in goats

Original languageEnglish
Pages (from-to)1629-1634
Number of pages6
JournalJournal of General Virology
Volume91
DOIs
Publication statusPublished - Jun 2010

Keywords

  • BREEDS
  • GENOTYPE
  • GOAT
  • PRP GENE
  • IDENTIFICATION
  • DISEASE
  • NATURAL SCRAPIE
  • SHEEP
  • PRION PROTEIN GENE

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