Capture of authentic embryonic stem cells from rat blastocysts

Mia Buehr; Stephen Meek; Kate Blair; Jian Yang; Janice Ure; Jose Silva; Renee McLay; John Hall; Qi-Long Ying; Austin Smith

doi:10.1016/j.cell.2008.12.007

Capture of authentic embryonic stem cells from rat blastocysts

Mia Buehr, Stephen Meek, Kate Blair, Jian Yang, Janice Ure, Jose Silva, Renee McLay, John Hall, Qi-Long Ying, Austin Smith

Research output: Contribution to journal › Article › peer-review

Abstract

Embryonic stem (ES) cells have been available from inbred mice since 1981 but have not been validated for other rodents. Failure to establish ES cells from a range of mammals challenges the identity of cultivated stem cells and our understanding of the pluripotent state. Here we investigated derivation of ES cells from the rat. We applied molecularly defined conditions designed to shield the ground state of authentic pluripotency from inductive differentiation stimuli. Undifferentiated cell lines developed that exhibited diagnostic features of ES cells including colonization of multiple tissues in viable chimeras. Definitive ES cell status was established by transmission of the cell line genome to offspring. Derivation of germline-competent ES cells from the rat paves the way to targeted genetic manipulation in this valuable biomedical model species. Rat ES cells will also provide a refined test-bed for functional evaluation of pluripotent stem cell-derived tissue repair and regeneration.

Original language	English
Pages (from-to)	1287-98
Number of pages	12
Journal	Cell
Volume	135
Issue number	7
DOIs	https://doi.org/10.1016/j.cell.2008.12.007
Publication status	Published - 26 Dec 2008

Keywords / Materials (for Non-textual outputs)

Animals
Blastocyst Inner Cell Mass
Cell Culture Techniques
Cell Line
Chimera
Embryonic Stem Cells
Female
Fibroblast Growth Factors
Glycogen Synthase Kinases
Male
Mice
Mice, SCID
Mitogen-Activated Protein Kinase Kinases
Rats
Rats, Inbred F344
Rats, Inbred Strains

Access to Document

10.1016/j.cell.2008.12.007

Cite this

@article{a0ab1e16ae044eac806407338513a202,

title = "Capture of authentic embryonic stem cells from rat blastocysts",

abstract = "Embryonic stem (ES) cells have been available from inbred mice since 1981 but have not been validated for other rodents. Failure to establish ES cells from a range of mammals challenges the identity of cultivated stem cells and our understanding of the pluripotent state. Here we investigated derivation of ES cells from the rat. We applied molecularly defined conditions designed to shield the ground state of authentic pluripotency from inductive differentiation stimuli. Undifferentiated cell lines developed that exhibited diagnostic features of ES cells including colonization of multiple tissues in viable chimeras. Definitive ES cell status was established by transmission of the cell line genome to offspring. Derivation of germline-competent ES cells from the rat paves the way to targeted genetic manipulation in this valuable biomedical model species. Rat ES cells will also provide a refined test-bed for functional evaluation of pluripotent stem cell-derived tissue repair and regeneration.",

keywords = "Animals, Blastocyst Inner Cell Mass, Cell Culture Techniques, Cell Line, Chimera, Embryonic Stem Cells, Female, Fibroblast Growth Factors, Glycogen Synthase Kinases, Male, Mice, Mice, SCID, Mitogen-Activated Protein Kinase Kinases, Rats, Rats, Inbred F344, Rats, Inbred Strains",

author = "Mia Buehr and Stephen Meek and Kate Blair and Jian Yang and Janice Ure and Jose Silva and Renee McLay and John Hall and Qi-Long Ying and Austin Smith",

year = "2008",

month = dec,

day = "26",

doi = "10.1016/j.cell.2008.12.007",

language = "English",

volume = "135",

pages = "1287--98",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier",

number = "7",

}

TY - JOUR

T1 - Capture of authentic embryonic stem cells from rat blastocysts

AU - Buehr, Mia

AU - Meek, Stephen

AU - Blair, Kate

AU - Yang, Jian

AU - Ure, Janice

AU - Silva, Jose

AU - McLay, Renee

AU - Hall, John

AU - Ying, Qi-Long

AU - Smith, Austin

PY - 2008/12/26

Y1 - 2008/12/26

N2 - Embryonic stem (ES) cells have been available from inbred mice since 1981 but have not been validated for other rodents. Failure to establish ES cells from a range of mammals challenges the identity of cultivated stem cells and our understanding of the pluripotent state. Here we investigated derivation of ES cells from the rat. We applied molecularly defined conditions designed to shield the ground state of authentic pluripotency from inductive differentiation stimuli. Undifferentiated cell lines developed that exhibited diagnostic features of ES cells including colonization of multiple tissues in viable chimeras. Definitive ES cell status was established by transmission of the cell line genome to offspring. Derivation of germline-competent ES cells from the rat paves the way to targeted genetic manipulation in this valuable biomedical model species. Rat ES cells will also provide a refined test-bed for functional evaluation of pluripotent stem cell-derived tissue repair and regeneration.

AB - Embryonic stem (ES) cells have been available from inbred mice since 1981 but have not been validated for other rodents. Failure to establish ES cells from a range of mammals challenges the identity of cultivated stem cells and our understanding of the pluripotent state. Here we investigated derivation of ES cells from the rat. We applied molecularly defined conditions designed to shield the ground state of authentic pluripotency from inductive differentiation stimuli. Undifferentiated cell lines developed that exhibited diagnostic features of ES cells including colonization of multiple tissues in viable chimeras. Definitive ES cell status was established by transmission of the cell line genome to offspring. Derivation of germline-competent ES cells from the rat paves the way to targeted genetic manipulation in this valuable biomedical model species. Rat ES cells will also provide a refined test-bed for functional evaluation of pluripotent stem cell-derived tissue repair and regeneration.

KW - Animals

KW - Blastocyst Inner Cell Mass

KW - Cell Culture Techniques

KW - Cell Line

KW - Chimera

KW - Embryonic Stem Cells

KW - Female

KW - Fibroblast Growth Factors

KW - Glycogen Synthase Kinases

KW - Male

KW - Mice

KW - Mice, SCID

KW - Mitogen-Activated Protein Kinase Kinases

KW - Rats

KW - Rats, Inbred F344

KW - Rats, Inbred Strains

U2 - 10.1016/j.cell.2008.12.007

DO - 10.1016/j.cell.2008.12.007

M3 - Article

C2 - 19109897

SN - 0092-8674

VL - 135

SP - 1287

EP - 1298

JO - Cell

JF - Cell

IS - 7

ER -

Capture of authentic embryonic stem cells from rat blastocysts

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Cite this