Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions

Max Crispin; Thomas A Bowden; Charlotte H Coles; Karl Harlos; Alexandru R Aricescu; D J Harvey; David I Stuart; E. Yvonne Jones

doi:10.1016/j.jmb.2009.02.033

Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions

Max Crispin, Thomas A Bowden, Charlotte H Coles, Karl Harlos, Alexandru R Aricescu, D J Harvey, David I Stuart, E. Yvonne Jones

Deanery of Clinical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Antibodies contain a conserved glycosylation site that has emerged as a target for the modulation of antibody effector functions. The crystal structure of a biosynthetic intermediate of human IgG1, bearing immature oligomannose-type glycans and reported to display increased antibody-dependent cellular cytotoxicity, demonstrates that glycan engineering can bias the Fc to an open conformation primed for receptor binding.

Original language	English
Pages (from-to)	1061-6
Number of pages	6
Journal	Journal of Molecular Biology
Volume	387
Issue number	5
DOIs	https://doi.org/10.1016/j.jmb.2009.02.033
Publication status	Published - 17 Apr 2009

Keywords / Materials (for Non-textual outputs)

Antibody-Dependent Cell Cytotoxicity
Carbohydrate Sequence
Crystallography, X-Ray
Glycosylation
Humans
Immunoglobulin Fc Fragments
Immunoglobulin G
Models, Molecular
Oligosaccharides
Protein Structure, Tertiary
Recombinant Proteins
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Static Electricity

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10.1016/j.jmb.2009.02.033

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title = "Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions",

abstract = "Antibodies contain a conserved glycosylation site that has emerged as a target for the modulation of antibody effector functions. The crystal structure of a biosynthetic intermediate of human IgG1, bearing immature oligomannose-type glycans and reported to display increased antibody-dependent cellular cytotoxicity, demonstrates that glycan engineering can bias the Fc to an open conformation primed for receptor binding.",

keywords = "Antibody-Dependent Cell Cytotoxicity, Carbohydrate Sequence, Crystallography, X-Ray, Glycosylation, Humans, Immunoglobulin Fc Fragments, Immunoglobulin G, Models, Molecular, Oligosaccharides, Protein Structure, Tertiary, Recombinant Proteins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Static Electricity",

author = "Max Crispin and Bowden, {Thomas A} and Coles, {Charlotte H} and Karl Harlos and Aricescu, {Alexandru R} and Harvey, {D J} and Stuart, {David I} and Jones, {E. Yvonne}",

year = "2009",

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doi = "10.1016/j.jmb.2009.02.033",

language = "English",

volume = "387",

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journal = "Journal of Molecular Biology",

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TY - JOUR

T1 - Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions

AU - Crispin, Max

AU - Bowden, Thomas A

AU - Coles, Charlotte H

AU - Harlos, Karl

AU - Aricescu, Alexandru R

AU - Harvey, D J

AU - Stuart, David I

AU - Jones, E. Yvonne

PY - 2009/4/17

Y1 - 2009/4/17

N2 - Antibodies contain a conserved glycosylation site that has emerged as a target for the modulation of antibody effector functions. The crystal structure of a biosynthetic intermediate of human IgG1, bearing immature oligomannose-type glycans and reported to display increased antibody-dependent cellular cytotoxicity, demonstrates that glycan engineering can bias the Fc to an open conformation primed for receptor binding.

AB - Antibodies contain a conserved glycosylation site that has emerged as a target for the modulation of antibody effector functions. The crystal structure of a biosynthetic intermediate of human IgG1, bearing immature oligomannose-type glycans and reported to display increased antibody-dependent cellular cytotoxicity, demonstrates that glycan engineering can bias the Fc to an open conformation primed for receptor binding.

KW - Antibody-Dependent Cell Cytotoxicity

KW - Carbohydrate Sequence

KW - Crystallography, X-Ray

KW - Glycosylation

KW - Humans

KW - Immunoglobulin Fc Fragments

KW - Immunoglobulin G

KW - Models, Molecular

KW - Oligosaccharides

KW - Protein Structure, Tertiary

KW - Recombinant Proteins

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Static Electricity

U2 - 10.1016/j.jmb.2009.02.033

DO - 10.1016/j.jmb.2009.02.033

M3 - Article

SN - 0022-2836

VL - 387

SP - 1061

EP - 1066

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 5

ER -

Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions

Abstract

Keywords / Materials (for Non-textual outputs)

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