Carriage of Streptococcus pneumoniae in aged adults with influenza-like-illness

Cassandra L. Krone, Anne L. Wyllie, Josine Van Beek, Nynke Y. Rots, Anna E. Oja, Mei Ling J N Chu, Jacob P. Bruin, Debby Bogaert, Elisabeth A M Sanders, Krzysztof Trzciñski

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Incidence of pneumococcal disease is disproportionally high in infants and elderly. Nasopharyngeal colonisation by Streptococcus pneumoniae is considered a prerequisite for disease but unlike in children, carriage in elderly is rarely detected. Here, we tested for S. pneumoniae in nasopharyngeal and saliva samples collected from community-dwelling elderly with influenza-like-illness (ILI). Trans-nasal nasopharyngeal, trans-oral nasopharyngeal and saliva samples (n = 270 per sample type) were collected during winter/spring 2011/2012 from 135 persons aged 60-89 at onset of ILI and 7-9 weeks later following recovery. After samples were tested for pneumococci by conventional culture, all plate growth was collected. DNA extracted from plate harvests was tested by quantitative-PCRs (qPCR) specific for S . pneumoniae and serotypes included in the 13-valent pneumococcal conjugated vaccine (PCV13). Pneumococci were cultured from 14 of 135 (10%) elderly with none of the sampled niches showing superiority in carriage detection. With 76/270 (28%) saliva, 31/270 (11%) trans-oral and 13/270 (5%) trans-nasal samples positive by qPCR, saliva was superior to nasopharyngeal swabs (p

Original languageEnglish
Article numbere0119875
JournalPLoS ONE
Issue number3
Publication statusPublished - 19 Mar 2015


Dive into the research topics of 'Carriage of Streptococcus pneumoniae in aged adults with influenza-like-illness'. Together they form a unique fingerprint.

Cite this