Cartilage gene expression correlates with radiographic severity of canine elbow osteoarthritis

Dylan N. Clements, Noel Fitzpatrick, Stuart D. Carter, Philip J. R. Day

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The relationship between radiographic measures of severity of osteoarthritis (OA) and changes in gene expression in joints are not well characterised. In this Study, the expression of 11 candidate genes was characterised by quantitative reverse transcriptase polymerase chain reaction in normal and OA cartilage and bone from the elbows of dogs with fragmented coronoid disease. The levels of expression of type I collagen alpha 2 chain (COL1A2), type III collagen alpha 1 chain (COL3A1]), lumican (LUM), matrix metalloproteinase-2 (AWN). -9 (MMP9) and -13 (,MMP13) genes were increased and the expression of tissue inhibitor of metalloproteinase-2 (TIMP2) and cathepsin D (CTSD) genes were decreased in OA cartilage relative to normal cartilage. All differences correlated with radiographic measures of severity of OA. Levels of expression of COL1A2, MMP2, MMP9, MMP13 and TIMP1 were increased, whereas expression of TIMP2 was decreased in OA bone relative to normal bone. Cartilage gene expression may be correlated with the radiographic severity of OA.

Original languageEnglish
Pages (from-to)211-218
Number of pages8
JournalThe Veterinary Journal
Issue number2
Publication statusPublished - Feb 2009

Keywords / Materials (for Non-textual outputs)

  • Osteoarthritis
  • Canine
  • Cartilage
  • Bone
  • Gene expression
  • Collagen
  • Lumican
  • Matrix metalloproteinase;
  • TIMP
  • Cathepsin


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