CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis

Matthew D. Smith, Margaret E Harley, Alain J Kemp, Jimi Wills, Martin Lee, Mark Arends, Alex von Kriegsheim, Christian Behrends, Simon Wilkinson

Research output: Contribution to journalArticlepeer-review

Abstract

Mechanisms of selective autophagy of the ER, known as ER-phagy, require molecular delineation, particularly in vivo. It is unclear how these events control ER proteostasis and cellular health. Here, we identify cell-cycle progression gene 1 (CCPG1), an ER-resident protein with no known physiological role, as a non-canonical cargo receptor that directly binds to core autophagy proteins via an LIR motif to mammalian ATG8 proteins and, independently and via a discrete motif, to FIP200. These interactions facilitate ER-phagy. The CCPG1 gene is inducible by the unfolded protein response and thus directly links ER stress to ER-phagy. In vivo, CCPG1 protects against ER luminal protein aggregation and consequent unfolded protein response hyperactivation and tissue injury of the exocrine pancreas. Thus, via identification of this autophagy protein, we describe an unexpected molecular mechanism of ER-phagy and provide evidence that this may be physiologically relevant in ER luminal proteostasis.
Original languageEnglish
Pages (from-to)P217-232.e11
JournalDevelopmental Cell
Volume44
Issue number2
Early online date28 Dec 2017
DOIs
Publication statusPublished - 22 Jan 2018

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