Projects per year
Abstract
The murine serous cavities contain a rare and enigmatic population of short-lived F4/80loMHCII+ macrophages but what regulates their development, survival and fate is unclear. Here, we show that mature F4/80loMHCII+ peritoneal macrophages arise post-birth, but that this occurs largely independently of colonisation by microbiota. Rather, microbiota specifically regulate development of a subpopulation of CD11c+ cells that express the immunoregulatory cytokine RELM, are reliant on the transcription factor EGR2, and develop independently of the growth factor CSF1. Furthermore, we demonstrate that intrinsic expression of RELM, a signature marker shared by CD11c+ and CD11c– F4/80loMHCII+ cavity macrophages, regulates survival and differentiation of these cells in the peritoneal cavity in a sex-specific manner. Thus, we identify a previously unappreciated diversity in serous cavity F4/80loMHCII+ macrophages that is regulated by microbiota, and describe a novel sex and site-specific function for RELM in regulating macrophage endurance that reveals the unique survival challenge presented to monocyte-derived macrophages by the female peritoneal environment.
Original language | English |
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Pages (from-to) | 1243-1257 |
Journal | European Journal of Immunology |
Volume | 52 |
Issue number | 8 |
Early online date | 24 May 2022 |
DOIs | |
Publication status | Published - 4 Aug 2022 |
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Dive into the research topics of 'CD11c identifies microbiota and EGR2-dependent MHCII+ serous cavity macrophages with sexually dimorphic fate in mice'. Together they form a unique fingerprint.Projects
- 3 Finished
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Wellcome Trust Tissue Repair PhD Programme MAIN AWARD
Forbes, S. (Principal Investigator), Hansen, C. (Co-investigator) & Rossi, A. (Co-investigator)
11/09/17 → 30/09/23
Project: Research
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Investigating the role of TGF¿¿ in the functional imprinting of pulmonary macrophages in health and disease
Bain, C. (Principal Investigator)
1/09/17 → 31/12/23
Project: Research
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The parameters of tissue resident macrophage autonomy
Jenkins, S. (Principal Investigator)
1/07/14 → 30/06/17
Project: Research
Equipment
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Institute for Regeneration and Repair Flow Cytometry Facility
Johnston, S. (Manager), Rossi, F. (Manager), Cryer, C. (Other) & Laird, A. (Other)
Institute of Regeneration and RepairFacility/equipment: Facility