CD148/DEP-1 association with areas of cytoskeletal organisation in macrophages

Richa K. Dave, David A. Hume, Caryn Elsegood, Stuart Kellie

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

In macrophages, tyrosine phosphorylation regulates many signalling pathways leading to growth, differentiation, activation, phagocytosis and adhesion. Protein tyrosine phosphatases (PTPs) represent a biochemical counterbalance to the activity of protein tyrosine kinases, thus regulating the dynamic phosphorylation state of a cell. CD148 is a receptor PTP that is highly expressed in macrophages and is further regulated by pro-inflammatory stimuli. CD148 is normally localised to the plasma membrane of macrophages. Following stimulation with CSF-1 or LPS, there was a re-distribution and concentration of CD148 in areas of membrane ruffling. Treatment of macrophages with anti-CD148 monoclonal antibody inhibited CSF-1-induced macrophage spreading, cytoskeletal re-arrangements and chemotaxis, without affecting cell survival. There were no detectable effects on the CSF-1 receptor-akt signalling pathway. These results are consistent with the hypothesis that CD148 is a regulator of macrophage activity.
Original languageEnglish
Pages (from-to)1734-1744
Number of pages11
JournalExperimental Cell Research
Issue number10
Publication statusPublished - 10 Jun 2009

Keywords / Materials (for Non-textual outputs)

  • Tyrosine phosphatase
  • Macrophage
  • Cytoskeleton


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