CD4-independent infection by HIV-2 (ROD/B): use of the 7-transmembrane receptors CXCR-4, CCR-3, and V28 for entry

J D Reeves, A McKnight, S Potempa, G Simmons, P W Gray, C A Power, T Wells, R A Weiss, Simon Talbot

Research output: Contribution to journalArticlepeer-review

Abstract

We have assayed a variety of 7tm chemokine receptors (CCR-2b, CCR-3, CCR-4, CCR-5, CXCR-1, CXCR-4) and two orphan 7tm receptors (V28 and EBI.1) for their ability to allow infection of CD4-negative feline kidney CCC cells by the HIV-2 strains ROD/A and ROD/B. We found that ROD/B was able to use CXCR-4 transiently expressed in CCC cells, and infection by ROD/A was enhanced 15-fold in the presence of sCD4. Feline CCC cells also became permissive to ROD/B and ROD/A entry when transiently transfected with the chemokine receptor CCR-3 or the orphan 7tm receptor V2B, when cultured in the presence of sCD4. Entry of ROD/A into CCC cells expressing CCR-3 could be blocked by 800 ng/ml eotaxin, the natural ligand for CCR-3.
Original languageEnglish
Pages (from-to)130-4
Number of pages5
JournalVirology
Volume231
Issue number1
DOIs
Publication statusPublished - 1997

Keywords

  • Animals
  • Antigens, CD4
  • Cats
  • Cell Line
  • Chemokine CCL11
  • Chemokines, CC
  • Chemotactic Factors, Eosinophil
  • Cytokines
  • HIV-2
  • Humans
  • Membrane Proteins
  • Receptors, CCR3
  • Receptors, CXCR4
  • Receptors, Chemokine
  • Receptors, Cytokine
  • Receptors, HIV

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