Cell culture of primary cerebellar granule cells

Dana Krämer, Liliana Minichiello

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract / Description of output

Cerebellar granule cells are often used as a model system for the study of neuronal development, function and pathology, including the analysis of activity-dependent survival/apoptosis of neurons and the mechanisms of neuroprotection. Cerebellar granule cells are generated postnatally and constitute the largest homogeneous neuronal population of the mammalian brain. In addition, cerebellar granule cells cultured in vitro develop characteristics of mature cerebellar granule cells seen in vivo, such as an extensive neuritic network, expression of excitatory amino acid receptors and production and release of -L: glutamate. Taken together, these features make cerebellar granule cells a unique model system that has been extensively characterised and used for in vitro studies.
Original languageEnglish
Title of host publicationMouse Cell Culture
PublisherHumana Press
Number of pages7
ISBN (Electronic)978-1-59745-019-5
ISBN (Print)978-1-58829-772-3
Publication statusPublished - 2010

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Keywords / Materials (for Non-textual outputs)

  • Mouse cerebellar granule cells
  • homogeneous neuronal cell culture
  • activity-dependent survival
  • central nervous system
  • postnatal development


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