Cell culture of primary cerebellar granule cells

Dana Krämer; Liliana Minichiello

doi:10.1007/978-1-59745-019-5_17

Cell culture of primary cerebellar granule cells

Dana Krämer, Liliana Minichiello

Deanery of Biomedical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed)

Abstract / Description of output

Cerebellar granule cells are often used as a model system for the study of neuronal development, function and pathology, including the analysis of activity-dependent survival/apoptosis of neurons and the mechanisms of neuroprotection. Cerebellar granule cells are generated postnatally and constitute the largest homogeneous neuronal population of the mammalian brain. In addition, cerebellar granule cells cultured in vitro develop characteristics of mature cerebellar granule cells seen in vivo, such as an extensive neuritic network, expression of excitatory amino acid receptors and production and release of -L: glutamate. Taken together, these features make cerebellar granule cells a unique model system that has been extensively characterised and used for in vitro studies.

Original language	English
Title of host publication	Mouse Cell Culture
Publisher	Humana Press
Pages	233-9
Number of pages	7
Volume	633
ISBN (Electronic)	978-1-59745-019-5
ISBN (Print)	978-1-58829-772-3
DOIs	https://doi.org/10.1007/978-1-59745-019-5_17
Publication status	Published - 2010

Publication series

Name	Methods in Molecular Biology
Volume	633
ISSN (Print)	1064-3745

Keywords / Materials (for Non-textual outputs)

Mouse cerebellar granule cells
homogeneous neuronal cell culture
activity-dependent survival
central nervous system
postnatal development

Access to Document

10.1007/978-1-59745-019-5_17

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title = "Cell culture of primary cerebellar granule cells",

abstract = "Cerebellar granule cells are often used as a model system for the study of neuronal development, function and pathology, including the analysis of activity-dependent survival/apoptosis of neurons and the mechanisms of neuroprotection. Cerebellar granule cells are generated postnatally and constitute the largest homogeneous neuronal population of the mammalian brain. In addition, cerebellar granule cells cultured in vitro develop characteristics of mature cerebellar granule cells seen in vivo, such as an extensive neuritic network, expression of excitatory amino acid receptors and production and release of -L: glutamate. Taken together, these features make cerebellar granule cells a unique model system that has been extensively characterised and used for in vitro studies.",

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T1 - Cell culture of primary cerebellar granule cells

AU - Krämer, Dana

AU - Minichiello, Liliana

PY - 2010

Y1 - 2010

N2 - Cerebellar granule cells are often used as a model system for the study of neuronal development, function and pathology, including the analysis of activity-dependent survival/apoptosis of neurons and the mechanisms of neuroprotection. Cerebellar granule cells are generated postnatally and constitute the largest homogeneous neuronal population of the mammalian brain. In addition, cerebellar granule cells cultured in vitro develop characteristics of mature cerebellar granule cells seen in vivo, such as an extensive neuritic network, expression of excitatory amino acid receptors and production and release of -L: glutamate. Taken together, these features make cerebellar granule cells a unique model system that has been extensively characterised and used for in vitro studies.

AB - Cerebellar granule cells are often used as a model system for the study of neuronal development, function and pathology, including the analysis of activity-dependent survival/apoptosis of neurons and the mechanisms of neuroprotection. Cerebellar granule cells are generated postnatally and constitute the largest homogeneous neuronal population of the mammalian brain. In addition, cerebellar granule cells cultured in vitro develop characteristics of mature cerebellar granule cells seen in vivo, such as an extensive neuritic network, expression of excitatory amino acid receptors and production and release of -L: glutamate. Taken together, these features make cerebellar granule cells a unique model system that has been extensively characterised and used for in vitro studies.

KW - Mouse cerebellar granule cells

KW - homogeneous neuronal cell culture

KW - activity-dependent survival

KW - central nervous system

KW - postnatal development

U2 - 10.1007/978-1-59745-019-5_17

DO - 10.1007/978-1-59745-019-5_17

M3 - Chapter (peer-reviewed)

C2 - 20204632

SN - 978-1-58829-772-3

VL - 633

T3 - Methods in Molecular Biology

SP - 233

EP - 239

BT - Mouse Cell Culture

PB - Humana Press

ER -

Cell culture of primary cerebellar granule cells

Abstract / Description of output

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Keywords / Materials (for Non-textual outputs)

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