Bromodeoxyuridine (BrdUrd) was incorporated into the DNA of proliferating chondrocytes of the tibiae and metatarsal growth plate of rats of differing ages. Immunodetection of the incorporated BrdUrd was achieved using a monoclonal antibody to BrdUrd and indirect immunofluorescence procedures. The labelling index within the growth plate was found to decrease with advancing age in the tibia and metatarsal. Glucose 6-phosphate dehydrogenase (G6PD) activity appeared to be related to chondrocyte proliferation as a significant correlation was observed between G6PD activity and the labelling index of the tibial chondrocytes. The results of this study indicate that G6PD activity is a possible marker for cartilage growth and also that the BrdUrd technique has several advantages over the conventional autoradiographic methods for the assessment of cell replication.
- Cell Division
- Fluorescent Antibody Technique
- Glucosephosphate Dehydrogenase
- Growth Plate