Cellular inhibitors of long interspersed element 1 and Alu retrotransposition

Hal P Bogerd; Heather L Wiegand; Amy E Hulme; José L Garcia-Perez; K Sue O'Shea; John V Moran; Bryan R Cullen

doi:10.1073/pnas.0603313103

Cellular inhibitors of long interspersed element 1 and Alu retrotransposition

Hal P Bogerd, Heather L Wiegand, Amy E Hulme, José L Garcia-Perez, K Sue O'Shea, John V Moran, Bryan R Cullen

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Long interspersed element (LINE) 1 retrotransposons are major genomic parasites that represent approximately 17% of the human genome. The LINE-1 ORF2 protein is also responsible for the mobility of Alu elements, which constitute a further approximately 11% of genomic DNA. Representative members of each element class remain mobile, and deleterious retrotransposition events can induce spontaneous genetic diseases. Here, we demonstrate that APOBEC3A and APOBEC3B, two members of the APOBEC3 family of human innate antiretroviral resistance factors, can enter the nucleus, where LINE-1 and Alu reverse transcription occurs, and specifically inhibit both LINE-1 and Alu retrotransposition. These data suggest that the APOBEC3 protein family may have evolved, at least in part, to defend the integrity of the human genome against endogenous retrotransposons.

Original language	English
Pages (from-to)	8780-5
Number of pages	6
Journal	Proceedings of the National Academy of Sciences (PNAS)
Volume	103
Issue number	23
DOIs	https://doi.org/10.1073/pnas.0603313103
Publication status	Published - 6 Jun 2006

Keywords / Materials (for Non-textual outputs)

Alu Elements
Cytosine Deaminase
DNA Transposable Elements
Gene Expression Regulation, Developmental
HeLa Cells
Humans
Long Interspersed Nucleotide Elements
Mutation
Protein Transport
RNA, Messenger

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10.1073/pnas.0603313103

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title = "Cellular inhibitors of long interspersed element 1 and Alu retrotransposition",

abstract = "Long interspersed element (LINE) 1 retrotransposons are major genomic parasites that represent approximately 17% of the human genome. The LINE-1 ORF2 protein is also responsible for the mobility of Alu elements, which constitute a further approximately 11% of genomic DNA. Representative members of each element class remain mobile, and deleterious retrotransposition events can induce spontaneous genetic diseases. Here, we demonstrate that APOBEC3A and APOBEC3B, two members of the APOBEC3 family of human innate antiretroviral resistance factors, can enter the nucleus, where LINE-1 and Alu reverse transcription occurs, and specifically inhibit both LINE-1 and Alu retrotransposition. These data suggest that the APOBEC3 protein family may have evolved, at least in part, to defend the integrity of the human genome against endogenous retrotransposons.",

keywords = "Alu Elements, Cytosine Deaminase, DNA Transposable Elements, Gene Expression Regulation, Developmental, HeLa Cells, Humans, Long Interspersed Nucleotide Elements, Mutation, Protein Transport, RNA, Messenger",

author = "Bogerd, {Hal P} and Wiegand, {Heather L} and Hulme, {Amy E} and Garcia-Perez, {Jos{\'e} L} and O'Shea, {K Sue} and Moran, {John V} and Cullen, {Bryan R}",

year = "2006",

month = jun,

day = "6",

doi = "10.1073/pnas.0603313103",

language = "English",

volume = "103",

pages = "8780--5",

journal = "Proceedings of the National Academy of Sciences (PNAS)",

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TY - JOUR

T1 - Cellular inhibitors of long interspersed element 1 and Alu retrotransposition

AU - Bogerd, Hal P

AU - Wiegand, Heather L

AU - Hulme, Amy E

AU - Garcia-Perez, José L

AU - O'Shea, K Sue

AU - Moran, John V

AU - Cullen, Bryan R

PY - 2006/6/6

Y1 - 2006/6/6

N2 - Long interspersed element (LINE) 1 retrotransposons are major genomic parasites that represent approximately 17% of the human genome. The LINE-1 ORF2 protein is also responsible for the mobility of Alu elements, which constitute a further approximately 11% of genomic DNA. Representative members of each element class remain mobile, and deleterious retrotransposition events can induce spontaneous genetic diseases. Here, we demonstrate that APOBEC3A and APOBEC3B, two members of the APOBEC3 family of human innate antiretroviral resistance factors, can enter the nucleus, where LINE-1 and Alu reverse transcription occurs, and specifically inhibit both LINE-1 and Alu retrotransposition. These data suggest that the APOBEC3 protein family may have evolved, at least in part, to defend the integrity of the human genome against endogenous retrotransposons.

AB - Long interspersed element (LINE) 1 retrotransposons are major genomic parasites that represent approximately 17% of the human genome. The LINE-1 ORF2 protein is also responsible for the mobility of Alu elements, which constitute a further approximately 11% of genomic DNA. Representative members of each element class remain mobile, and deleterious retrotransposition events can induce spontaneous genetic diseases. Here, we demonstrate that APOBEC3A and APOBEC3B, two members of the APOBEC3 family of human innate antiretroviral resistance factors, can enter the nucleus, where LINE-1 and Alu reverse transcription occurs, and specifically inhibit both LINE-1 and Alu retrotransposition. These data suggest that the APOBEC3 protein family may have evolved, at least in part, to defend the integrity of the human genome against endogenous retrotransposons.

KW - Alu Elements

KW - Cytosine Deaminase

KW - DNA Transposable Elements

KW - Gene Expression Regulation, Developmental

KW - HeLa Cells

KW - Humans

KW - Long Interspersed Nucleotide Elements

KW - Mutation

KW - Protein Transport

KW - RNA, Messenger

U2 - 10.1073/pnas.0603313103

DO - 10.1073/pnas.0603313103

M3 - Article

C2 - 16728505

SN - 0027-8424

VL - 103

SP - 8780

EP - 8785

JO - Proceedings of the National Academy of Sciences (PNAS)

JF - Proceedings of the National Academy of Sciences (PNAS)

IS - 23

ER -

Cellular inhibitors of long interspersed element 1 and Alu retrotransposition

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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